Therapeutic effects of bone marrow-derived mesenchymal stem cells on radiation-induced lung injury

  • Authors:
    • Chengcheng Xia
    • Pengyu Chang
    • Yuyu Zhang
    • Weiyan Shi
    • Bin Liu
    • Lijuan Ding
    • Min Liu
    • Ling Gao
    • Lihua Dong
  • View Affiliations

  • Published online on: November 17, 2015     https://doi.org/10.3892/or.2015.4433
  • Pages: 731-738
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Abstract

Radiation-induced lung injury (RILI) is a fatal condition featured by interstitial pneumonitis and fibrosis. Mesenchymal stem cells (MSCs) have been widely used for treating RILI in rodent models. In the present study, we aimed to investigate whether the therapeutic effects of human bone marrow-derived mesenchymal stem cells (hBM-MSCs) on RILI were in a dose-dependent manner. A total of 100 mice were randomly divided into: a control group (n=25), subject to lung irradiation and injection of phosphate-buffered solution (PBS) via the tail vein; and the hBM-MSC group, subject to lung irradiation followed by injection of a low dose (1x103 hBM-MSCs/g), medium dose (5x103 hBM-MSCs/g) and high dose (1x104 hBM-MSCs/g) of hBM-MSCs in PBS through the tail vein, respectively. After sacrifice, the pulmonary tissues were subject to hematoxylin and eosin (H&E) staining, Masson's trichrome staining and immunohistochemical staining to investigate the pathological changes. Immunofluorescent staining was performed to evaluate the differentiation capacity of hBM-MSCs in vivo by analyzing the expression of SPC and PECAM. hBM-MSCs improved the survival rate and histopathological features in the irradiated mice, especially in the low-dose group. Marked decrease in collagen deposition was noted in the irradiated mice treated using a low dose of hBM-MSCs. In addition, hBM-MSCs attenuated secretion and expression of IL-10 and increased the expression of TNF-α. Furthermore, hBM-MSCs had the potential to differentiate into functional cells upon lung injury. Low-dose hBM-MSCs contributed to functional recovery in mice with RILI.
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February-2016
Volume 35 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Xia C, Chang P, Zhang Y, Shi W, Liu B, Ding L, Liu M, Gao L and Dong L: Therapeutic effects of bone marrow-derived mesenchymal stem cells on radiation-induced lung injury. Oncol Rep 35: 731-738, 2016
APA
Xia, C., Chang, P., Zhang, Y., Shi, W., Liu, B., Ding, L. ... Dong, L. (2016). Therapeutic effects of bone marrow-derived mesenchymal stem cells on radiation-induced lung injury. Oncology Reports, 35, 731-738. https://doi.org/10.3892/or.2015.4433
MLA
Xia, C., Chang, P., Zhang, Y., Shi, W., Liu, B., Ding, L., Liu, M., Gao, L., Dong, L."Therapeutic effects of bone marrow-derived mesenchymal stem cells on radiation-induced lung injury". Oncology Reports 35.2 (2016): 731-738.
Chicago
Xia, C., Chang, P., Zhang, Y., Shi, W., Liu, B., Ding, L., Liu, M., Gao, L., Dong, L."Therapeutic effects of bone marrow-derived mesenchymal stem cells on radiation-induced lung injury". Oncology Reports 35, no. 2 (2016): 731-738. https://doi.org/10.3892/or.2015.4433