Significance of Lysyl oxidase‑like 2 gene expression on the epithelial‑mesenchymal status of hepatocellular carcinoma

  • Authors:
    • Go Ninomiya
    • Suguru Yamada
    • Masamichi Hayashi
    • Shigeomi Takeda
    • Masaya Suenaga
    • Hideki Takami
    • Mitsuro Kanda
    • Naoki Iwata
    • Yukiko Niwa
    • Chie Tanaka
    • Daisuke Kobayashi
    • Tsutomu Fujii
    • Goro Nakayama
    • Hiroyuki Sugimoto
    • Masahiko Koike
    • Michitaka Fujiwara
    • Yasuhiro Kodera
  • View Affiliations

  • Published online on: April 2, 2018     https://doi.org/10.3892/or.2018.6349
  • Pages: 2664-2672
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Abstract

In the present study, we investigated the role of lysyl oxidase‑like 2 (LOXL2), the correlation between LOXL2 and epithelial to mesenchymal transition (EMT) and the effects of using β‑aminopropionitrile (BAPN) to inhibit LOXL2 with the aim of reducing tumor progression in hepatocellular carcinoma (HCC). The expression level of LOXL2 was evaluated in HCC and adjacent non‑cancerous tissues using quantitative reverse transcription polymerase chain reaction and clinicopathological analyses. The effects of BAPN on cell proliferation, migration and invasion were investigated in vitro. Additionally, LOXL2 expression was assessed in the culture supernatants of HCC cell lines. Our results revealed that LOXL2 expression was higher in HCC cell lines and tissues. There was a significant correlation between EMT status and LOXL2 levels (P=0.004). BAPN reduced migration and invasion in HCC cells. HCC patients with high levels of LOXL2 expression had relatively shorter disease‑free survival (P=0.009) and overall survival (P=0.035). The expression level of LOXL2 was similar between cell supernatants and HCC cell lines. A multivariate analysis demonstrated that portal vein invasion (P=0.015), venous invasion (P=0.026), serum AFP (α‑fetoprotein) levels (P=0.019) and LOXL2 expression (P=0.009) were independent prognostic factors. Our results indicated that a higher level of LOXL2 may contribute to tumor progression, indicating that LOXL2 has clinical value as a therapeutic target in HCC.
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June-2018
Volume 39 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Ninomiya G, Yamada S, Hayashi M, Takeda S, Suenaga M, Takami H, Kanda M, Iwata N, Niwa Y, Tanaka C, Tanaka C, et al: Significance of Lysyl oxidase‑like 2 gene expression on the epithelial‑mesenchymal status of hepatocellular carcinoma. Oncol Rep 39: 2664-2672, 2018
APA
Ninomiya, G., Yamada, S., Hayashi, M., Takeda, S., Suenaga, M., Takami, H. ... Kodera, Y. (2018). Significance of Lysyl oxidase‑like 2 gene expression on the epithelial‑mesenchymal status of hepatocellular carcinoma. Oncology Reports, 39, 2664-2672. https://doi.org/10.3892/or.2018.6349
MLA
Ninomiya, G., Yamada, S., Hayashi, M., Takeda, S., Suenaga, M., Takami, H., Kanda, M., Iwata, N., Niwa, Y., Tanaka, C., Kobayashi, D., Fujii, T., Nakayama, G., Sugimoto, H., Koike, M., Fujiwara, M., Kodera, Y."Significance of Lysyl oxidase‑like 2 gene expression on the epithelial‑mesenchymal status of hepatocellular carcinoma". Oncology Reports 39.6 (2018): 2664-2672.
Chicago
Ninomiya, G., Yamada, S., Hayashi, M., Takeda, S., Suenaga, M., Takami, H., Kanda, M., Iwata, N., Niwa, Y., Tanaka, C., Kobayashi, D., Fujii, T., Nakayama, G., Sugimoto, H., Koike, M., Fujiwara, M., Kodera, Y."Significance of Lysyl oxidase‑like 2 gene expression on the epithelial‑mesenchymal status of hepatocellular carcinoma". Oncology Reports 39, no. 6 (2018): 2664-2672. https://doi.org/10.3892/or.2018.6349