Let-7a inhibits growth and migration of breast cancer cells by targeting HMGA1

  • Authors:
    • Kui Liu
    • Chunfu Zhang
    • Tao Li
    • Yanling Ding
    • Tao Tu
    • Fangfang Zhou
    • Wenkai Qi
    • Huabiao Chen
    • Xiaochun Sun
  • View Affiliations

  • Published online on: April 1, 2015     https://doi.org/10.3892/ijo.2015.2949
  • Pages: 2526-2534
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Abstract

Let-7 is one of the earliest discovered microRNAs (miRNAs) and has been reported to regulate self renewal and tumorigenicity of breast cancer cells. Let-7a is a member of this family and its function has not been fully characterized in breast cancer. First, total RNAs of breast cancer cells (MDA-MB-231, MCF-7), breast cancer tissues and corresponding adjacent normal tissues were extracted and used to detect let-7a expression by qRT-PCR. Secondly, the effects of let-7a on proliferation, colony formation, migration and invasion of breast cancer cells were assessed by in vitro cell culture experiments. Finally, western blotting was performed to demonstrate how let-7a regulated HMGA1 expression. We found that let-7a expression was significantly lower in breast cancer cells and breast cancer tissues compared to corresponding adjacent normal tissues. Cell proliferation, colony formation, migration and invasion were decreased after overexpression of let-7a in breast cancer cells and vice versa. Furthermore, we identified the high mobility group A1 (HMGA1) as a potential target gene of let-7a. Protein expression of the target gene was significantly downregulated in let-7a mimic transfected breast cancer cells and significantly upregulated in let-7a inhibitor transfected breast cancer cells. Our data suggest that let-7a plays an important role as a tumor suppressor gene by targeting HMGA1, which may open novel perspectives for clinical treatments against breast cancer.
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June-2015
Volume 46 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Liu K, Zhang C, Li T, Ding Y, Tu T, Zhou F, Qi W, Chen H and Sun X: Let-7a inhibits growth and migration of breast cancer cells by targeting HMGA1. Int J Oncol 46: 2526-2534, 2015
APA
Liu, K., Zhang, C., Li, T., Ding, Y., Tu, T., Zhou, F. ... Sun, X. (2015). Let-7a inhibits growth and migration of breast cancer cells by targeting HMGA1. International Journal of Oncology, 46, 2526-2534. https://doi.org/10.3892/ijo.2015.2949
MLA
Liu, K., Zhang, C., Li, T., Ding, Y., Tu, T., Zhou, F., Qi, W., Chen, H., Sun, X."Let-7a inhibits growth and migration of breast cancer cells by targeting HMGA1". International Journal of Oncology 46.6 (2015): 2526-2534.
Chicago
Liu, K., Zhang, C., Li, T., Ding, Y., Tu, T., Zhou, F., Qi, W., Chen, H., Sun, X."Let-7a inhibits growth and migration of breast cancer cells by targeting HMGA1". International Journal of Oncology 46, no. 6 (2015): 2526-2534. https://doi.org/10.3892/ijo.2015.2949