Characteristics of carbonic anhydrase 9 expressing cells in human intestinal crypt base

  • Authors:
    • Hidekazu Takahashi
    • Yozo Suzuki
    • Junichi Nishimura
    • Naotsugu Haraguchi
    • Masahisa Ohtsuka
    • Susumu Miyazaki
    • Mamoru Uemura
    • Taishi Hata
    • Ichiro Takemasa
    • Tsunekazu Mizushima
    • Hirofumi Yamamoto
    • Yuichiro Doki
    • Masaki Mori
  • View Affiliations

  • Published online on: November 20, 2015     https://doi.org/10.3892/ijo.2015.3260
  • Pages: 115-122
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Abstract

Though recent studies have revealed that stem cells of many tissues are harbored in hypoxic microenvironment, little is known about the relationship between hypoxia and intestinal crypt base, where intestinal stem cells are supposed to exist. In this study, we focused on carbonic anhydrase IX (CA9), a hypoxia-inducible membrane-tethered protein, in normal intestinal crypt base, adenoma and early colorectal cancer. Using surgically resected human colorectal cancer specimen, we searched for the expression pattern and functional association of CA9 in human adult normal intestinal epithelia, adenoma and early colorectal cancer by immunofluorescent and immunohistochemical staining, flow cytometry, and quantitative real-time-polymerase chain reaction. We demonstrated that almost all crypt base slender cells in ileum and crypt base cells with eosinophilic structure in their basal cytoplasm in right and left colon were CA9+ with the ratio of 25 to 40%, and that adenoma and T1 colorectal cancer showed broad expression of CA9. Flow cytometrically sorted CA9+ population showed increased mRNA level of a Wnt signaling factor AXIN2. In conclusion, these observations indicate that CA9 expression in normal crypt base cells has association with intestinal epithelial stemness and CA9 may be involved in the carcinogenesis of colorectal cancer.
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January-2016
Volume 48 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Takahashi H, Suzuki Y, Nishimura J, Haraguchi N, Ohtsuka M, Miyazaki S, Uemura M, Hata T, Takemasa I, Mizushima T, Mizushima T, et al: Characteristics of carbonic anhydrase 9 expressing cells in human intestinal crypt base. Int J Oncol 48: 115-122, 2016
APA
Takahashi, H., Suzuki, Y., Nishimura, J., Haraguchi, N., Ohtsuka, M., Miyazaki, S. ... Mori, M. (2016). Characteristics of carbonic anhydrase 9 expressing cells in human intestinal crypt base. International Journal of Oncology, 48, 115-122. https://doi.org/10.3892/ijo.2015.3260
MLA
Takahashi, H., Suzuki, Y., Nishimura, J., Haraguchi, N., Ohtsuka, M., Miyazaki, S., Uemura, M., Hata, T., Takemasa, I., Mizushima, T., Yamamoto, H., Doki, Y., Mori, M."Characteristics of carbonic anhydrase 9 expressing cells in human intestinal crypt base". International Journal of Oncology 48.1 (2016): 115-122.
Chicago
Takahashi, H., Suzuki, Y., Nishimura, J., Haraguchi, N., Ohtsuka, M., Miyazaki, S., Uemura, M., Hata, T., Takemasa, I., Mizushima, T., Yamamoto, H., Doki, Y., Mori, M."Characteristics of carbonic anhydrase 9 expressing cells in human intestinal crypt base". International Journal of Oncology 48, no. 1 (2016): 115-122. https://doi.org/10.3892/ijo.2015.3260