Serum anti-osteopontin autoantibody as a novel diagnostic and prognostic biomarker in patients with hepatocellular carcinoma

  • Authors:
    • Xia Ying
    • Yue Zhao
    • Jun-Lan Wang
    • Xia Zhou
    • Jing Zhao
    • Chen-Chen He
    • Xi-Jing Guo
    • Gui-Hua Jin
    • Li-Juan Wang
    • Qing Zhu
    • Su-Xia Han
  • View Affiliations

  • Published online on: July 30, 2014     https://doi.org/10.3892/or.2014.3367
  • Pages: 1550-1556
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Abstract

Osteopontin (OPN) is a secreted phosphorylated and glycosylated protein, which plays an important role in carcinogenesis and metastasis. In hepatocellular carcinoma (HCC), OPN is being investigated either as a therapeutic target gene or as a biomarker for diagnosis. Yet, the role of the anti-OPN autoantibody in HCC remains unclear. In the present study, the level of serum anti-OPN autoantibody in HCC was analyzed by enzyme-linked immunosorbent assay. Immunohistochemistry (IHC) was also performed to analyze protein expression profiles and the prognostic significance of OPN in HCC. In this study, the prevalence and titer of anti-OPN autoantibodies in HCC were significantly higher than these values in normal human serum (NHS) (P=0.001, P=0.000, respectively). When both α-fetoprotein and the autoantibody against OPN were used simultaneously as diagnostic biomarkers, the sensitivity was up to 65%. In IHC, 59 of the 83 (65.6%) HCC specimens expressed OPN with cytoplasmic positive staining. The overall survival (OS) of HCC patients with OPN-positive tumors was 28.81 months compared to 39.37 months for HCC patients with OPN-negative tumors (P<0.01). Furthermore, multivariate analysis showed that OPN overexpression was the strongest independent adverse prognostic factor for OS (P=0.02). Taken together, our data indicate that the anti-OPN autoantibody may be a supplementary serological biomarker for HCC, and is correlated with poor prognosis in HCC patients.
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October 2014
Volume 32 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Ying X, Zhao Y, Wang J, Zhou X, Zhao J, He C, Guo X, Jin G, Wang L, Zhu Q, Zhu Q, et al: Serum anti-osteopontin autoantibody as a novel diagnostic and prognostic biomarker in patients with hepatocellular carcinoma. Oncol Rep 32: 1550-1556, 2014
APA
Ying, X., Zhao, Y., Wang, J., Zhou, X., Zhao, J., He, C. ... Han, S. (2014). Serum anti-osteopontin autoantibody as a novel diagnostic and prognostic biomarker in patients with hepatocellular carcinoma. Oncology Reports, 32, 1550-1556. https://doi.org/10.3892/or.2014.3367
MLA
Ying, X., Zhao, Y., Wang, J., Zhou, X., Zhao, J., He, C., Guo, X., Jin, G., Wang, L., Zhu, Q., Han, S."Serum anti-osteopontin autoantibody as a novel diagnostic and prognostic biomarker in patients with hepatocellular carcinoma". Oncology Reports 32.4 (2014): 1550-1556.
Chicago
Ying, X., Zhao, Y., Wang, J., Zhou, X., Zhao, J., He, C., Guo, X., Jin, G., Wang, L., Zhu, Q., Han, S."Serum anti-osteopontin autoantibody as a novel diagnostic and prognostic biomarker in patients with hepatocellular carcinoma". Oncology Reports 32, no. 4 (2014): 1550-1556. https://doi.org/10.3892/or.2014.3367