Vaccination with OVA-bound nanoparticles encapsulating IL-7 inhibits the growth of OVA-expressing E.G7 tumor cells in vivo

  • Authors:
    • Hiroko Toyota
    • Noriko Yanase
    • Takayuki Yoshimoto
    • Mitsunori Harada
    • Yasuki Kato
    • Junichiro Mizuguchi
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  • Published online on: November 12, 2014     https://doi.org/10.3892/or.2014.3603
  • Pages: 292-296
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Abstract

Immunotherapy has gained special attention due to its specific effects on tumor cells and systemic action to block metastasis. We recently demonstrated that ovalbumin (OVA) conjugated to the surface of nanoparticles (NPs) (OVA‑NPs) can manipulate humoral immune responses. In the present study, we aimed to ascertain whether vaccination with OVA-NPs entrapping IL-7 (OVA-NPs-IL-7) are able to induce antitumor immune responses in vivo. Pretreatment with a subcutaneous inoculation of OVA-NPs delayed the growth of thymic lymphoma cells expressing a model tumor antigen OVA (E.G7-OVA), and OVA-NPs-IL-7 substantially blocked the growth of E.G7-OVA tumor cells, although NPs-IL-7 alone had a meager effect, as assessed by the mean tumor size and the percentage of tumor-free mice. However, pretreatment with OVA-NPs-IL-7 failed to reduce the growth of parental thymic tumor cells, suggesting that the antitumor effect was antigen-specific. A tetramer assay revealed that vaccination with OVA-NPs-IL-7 tended to enhance the proportion of cytotoxic T cells (CTLs) specific for OVA. When the tumor-free mice inoculated with OVA-NPs-IL-7 plus EG.7 cells were rechallenged with E.G7-OVA cells, they demonstrated reduced growth compared with that in the control mice. Thus, a single subcutaneous injection of OVA-NPs-IL-7 into mice induced tumor-specific and also memory-like immune responses, resulting in regression of tumor cells. Antigens on NPs entrapping IL-7 would be a promising carrier to develop and enhance immune responses, including humoral and cellular immunity as well as a method of drug delivery to a specific target of interest.
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January-2015
Volume 33 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Toyota H, Yanase N, Yoshimoto T, Harada M, Kato Y and Mizuguchi J: Vaccination with OVA-bound nanoparticles encapsulating IL-7 inhibits the growth of OVA-expressing E.G7 tumor cells in vivo. Oncol Rep 33: 292-296, 2015.
APA
Toyota, H., Yanase, N., Yoshimoto, T., Harada, M., Kato, Y., & Mizuguchi, J. (2015). Vaccination with OVA-bound nanoparticles encapsulating IL-7 inhibits the growth of OVA-expressing E.G7 tumor cells in vivo. Oncology Reports, 33, 292-296. https://doi.org/10.3892/or.2014.3603
MLA
Toyota, H., Yanase, N., Yoshimoto, T., Harada, M., Kato, Y., Mizuguchi, J."Vaccination with OVA-bound nanoparticles encapsulating IL-7 inhibits the growth of OVA-expressing E.G7 tumor cells in vivo". Oncology Reports 33.1 (2015): 292-296.
Chicago
Toyota, H., Yanase, N., Yoshimoto, T., Harada, M., Kato, Y., Mizuguchi, J."Vaccination with OVA-bound nanoparticles encapsulating IL-7 inhibits the growth of OVA-expressing E.G7 tumor cells in vivo". Oncology Reports 33, no. 1 (2015): 292-296. https://doi.org/10.3892/or.2014.3603