Open Access

Green tea polyphenol epigallocatechin-O-gallate induces cell death by acid sphingomyelinase activation in chronic myeloid leukemia cells

  • Authors:
    • Yuhui Huang
    • Motofumi Kumazoe
    • Jaehoon Bae
    • Shuhei Yamada
    • Mika Takai
    • Shiori Hidaka
    • Shuya Yamashita
    • Yoonhee Kim
    • Yeongseon Won
    • Motoki Murata
    • Shuntaro Tsukamoto
    • Hirofumi Tachibana
  • View Affiliations

  • Published online on: June 26, 2015     https://doi.org/10.3892/or.2015.4086
  • Pages: 1162-1168
  • Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

An epidemiological study showed that green tea consumption is associated with a reduced risk of hematopoietic malignancy. The major green tea polyphenol epigallocatechin‑3-O-gallate (EGCG) is reported to have anticancer effects. Chronic myeloid leukemia (CML) is a major hematopoietic malignancy characterized by expansion of myeloid cells. In the present study, we showed EGCG-induced acid sphingomyelinase (ASM) activation and lipid raft clustering in CML cells. The ASM inhibitor desipramine significantly reduced EGCG-induced cell death. Protein kinase Cδ is a well‑known kinase that plays an important role in ASM activation. We observed EGCG-induced phosphorylation of protein kinase Cδ at Ser664. Importantly, EGCG-induced ASM activation was significantly reduced by pretreatment of CML cells with the soluble guanylate cyclase inhibitor NS2028, suggesting that EGCG induced ASM activation through the cyclic guanosine monophosphate (cGMP)-dependent pathway. Indeed, pharmacological inhibition of a cGMP-negative regulator enhanced the anti-CML effect of EGCG. These results indicate that EGCG-induced cell death via the cGMP/ASM pathway in CML cells.
View Figures
View References

Related Articles

Journal Cover

September-2015
Volume 34 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Huang Y, Kumazoe M, Bae J, Yamada S, Takai M, Hidaka S, Yamashita S, Kim Y, Won Y, Murata M, Murata M, et al: Green tea polyphenol epigallocatechin-O-gallate induces cell death by acid sphingomyelinase activation in chronic myeloid leukemia cells. Oncol Rep 34: 1162-1168, 2015.
APA
Huang, Y., Kumazoe, M., Bae, J., Yamada, S., Takai, M., Hidaka, S. ... Tachibana, H. (2015). Green tea polyphenol epigallocatechin-O-gallate induces cell death by acid sphingomyelinase activation in chronic myeloid leukemia cells. Oncology Reports, 34, 1162-1168. https://doi.org/10.3892/or.2015.4086
MLA
Huang, Y., Kumazoe, M., Bae, J., Yamada, S., Takai, M., Hidaka, S., Yamashita, S., Kim, Y., Won, Y., Murata, M., Tsukamoto, S., Tachibana, H."Green tea polyphenol epigallocatechin-O-gallate induces cell death by acid sphingomyelinase activation in chronic myeloid leukemia cells". Oncology Reports 34.3 (2015): 1162-1168.
Chicago
Huang, Y., Kumazoe, M., Bae, J., Yamada, S., Takai, M., Hidaka, S., Yamashita, S., Kim, Y., Won, Y., Murata, M., Tsukamoto, S., Tachibana, H."Green tea polyphenol epigallocatechin-O-gallate induces cell death by acid sphingomyelinase activation in chronic myeloid leukemia cells". Oncology Reports 34, no. 3 (2015): 1162-1168. https://doi.org/10.3892/or.2015.4086