Characterization of insulin-producing cells derived from PDX-1-transfected neural stem cells

Wang,Hailan; Jiang,Zesheng; Li,Aihui; Gao,Yi

doi:10.3892/mmr.2012.1089

Characterization of insulin-producing cells derived from PDX-1-transfected neural stem cells

Authors:
- Hailan Wang
- Zesheng Jiang
- Aihui Li
- Yi Gao
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Affiliations: Department of Endocrinology, Shenzhen Longgang Central Hospital, Shenzhen 518000, P.R. China, Department of General Surgery, Zhujiang Hospital of South Medical University, Guangzhou 510120, P.R. China, Department of General Surgery, Dongguan Donghua Hospital, Dongguan 523000, P.R. China
Published online on: September 20, 2012 https://doi.org/10.3892/mmr.2012.1089
Pages: 1428-1432

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Abstract

Islet cell transplantation is a promising treatment strategy for type-1 diabetes. However, functional islet cells are hard to obtain for transplantation and are in short supply. Directing the differentiation of stem cells into insulin‑producing cells, which serve as islet cells, would overcome this shortage. Bone marrow contains hematopoietic stem cells and mesenchymal stem cells. The present study used bone marrow cells isolated from rats and neural stem cells (NSCs) that were derived from bone marrow cells in culture. Strong nestin staining was detected in NSCs, but not in bone marrow stromal cells (BMSCs). In vitro transfection of the pancreatic duodenal homeobox-1 (PDX-1) gene into NSCs generated insulin‑producing cells. Reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) analysis confirmed that PDX-1-transfected NSCs expressed insulin mRNA and released insulin protein. However, insulin release from PDX-1-transfected NSCs did not respond to the challenge of glucose and glucagon-like peptide-1. These results support the use of bone marrow-derived NSCs as a renewable source of insulin-producing cells for autologous transplantation to treat type-1 diabetes.

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