Effects of SASH1 on melanoma cell proliferation and apoptosis in vitro

Lin,Sheyu; Zhang,Junyu; Xu,Jiawei; Wang,Honglian; Sang,Qing; Xing,Qinghe; He,Lin

doi:10.3892/mmr.2012.1099

Effects of SASH1 on melanoma cell proliferation and apoptosis in vitro

Authors:
- Sheyu Lin
- Junyu Zhang
- Jiawei Xu
- Honglian Wang
- Qing Sang
- Qinghe Xing
- Lin He
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Affiliations: School of Life Sciences, Nantong University, Nantong 226019, P.R. China, Children's Hospital, Institute of Biomedical Sciences, Fudan University, Shanghai 200032, P.R. China
Published online on: September 26, 2012 https://doi.org/10.3892/mmr.2012.1099
Pages: 1243-1248

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Abstract

The SAM and SH3 domain containing 1 (SASH1) gene was originally identified as a potential tumor suppressor gene in breast cancer, mapped on chromosome 6q24.3. The expression of SASH1 plays a prognostic role in human colon cancer. Its expression is frequently downregulated in several human malignancies. However, the biological function of SASH1 in melanoma cells is yet to be determined. In this study, in order to investigate the tumor suppressive effects of the SASH1 gene, an A-375 stable melanoma cell line was established, overexpressing the SASH1 gene. The stable cell line was examined using proliferation assay, apoptosis assay, cell cycle analysis and real-time PCR. The results indicated that the tumor suppressive activity of SASH1 derived from G2/M arrest in A-375 cells, and that the phosphorylation of Cdc2 or the disruption of cyclin B-Cdc2 binding may be responsible for the G2/M arrest.

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