Effect of serum from sub-healthy subjects with fatigue on the gene expression profile of skeletal muscle cells

Li,Bao-Liang; Zhao,Xiao-Shan; Sun,Xiao-Min; Li,Jun; Chen,Jing; Luo,Ren; Zhang,Qi

doi:10.3892/mmr.2012.1219

Effect of serum from sub-healthy subjects with fatigue on the gene expression profile of skeletal muscle cells

Authors:
- Bao-Liang Li
- Xiao-Shan Zhao
- Xiao-Min Sun
- Jun Li
- Jing Chen
- Ren Luo
- Qi Zhang
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Affiliations: Department of Digestive Diseases, Changzhou Hospital of TCM, Changzhou 213003, P.R. China, Department of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P.R. China
Published online on: December 3, 2012 https://doi.org/10.3892/mmr.2012.1219
Pages: 454-460

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Abstract

To investigate the effect of serum from sub-healthy subjects with fatigue on the gene expression profile of skeletal muscle cells (HSkMCs), HSkMCs were cultured in vitro and treated with serum from healthy and sub-healthy subjects independently. Total RNA was extracted, purified and used for reverse transcription into cDNA. This was labeled with one of two fluorescence dyes, Cy3 or Cy5, and hybridized with a Human Genome Array carrying 8064 target genes. The differentially expressed genes were selected with the aid of software. There were 86 differentially expressed genes identified between the sub-healthy subjects and the healthy individuals, of which 60 were upregulated and 26 were downregulated compared with those of the healthy individuals. These genes were mainly associated with the cell cycle, membrane channels, protein transport, energy metabolism and apoptosis. Our results suggest that serum from sub-healthy subjects with fatigue may change the gene expression profile of HSkMCs.

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1	Christensen T, Bendix T and Kehlet H: Fatigue and cardiorespiratory function following abdominal surgery. Br J Surg. 69:417–9. 1982. View Article : Google Scholar : PubMed/NCBI
2	Greenberg MJ, Mealy TR, Jones M, Szczesna-Cordary D and Moore JR: The direct molecular effects of fatigue and myosin regulatory light chain phosphorylation on the actomyosin contractile apparatus. Am J Physiol Regul Integr Comp Physiol. 298:R989–R996. 2010. View Article : Google Scholar
3	Minshull C, Eston R, Rees D and Gleeson N: Knee joint neuromuscular activation performance during muscle damage and superimposed fatigue. J Sports Sci. 30:1015–1024. 2012. View Article : Google Scholar : PubMed/NCBI
4	Alessio HM: Exercise-induced oxdative stress. Med Sci Sports Exerc. 25:218–224. 1993. View Article : Google Scholar : PubMed/NCBI
5	Zhang Y, Wen L, Nie JL, et al: Study on molecular mechanism of exercise-induced fatigue in mitochondrial membrane III. Relationships between proton potential energy across membrane and generation of free radicals during acute exercise. Chin J Sports Med. 19:346–349. 2000.
6	Zhang YL and Chen JT: Effects of taurine on free radical metabolism, membrane fluidity and calcium transfer after chronic exercise in rats. Chin J Sports Med. 18:248–251. 1999.
7	Li BL, Zhao XS, Luo R, et al: Influence of sub-health fatigue serum on the activity of cytochrome C oxidase and energy charge of mitochondrial membrane of human skeletal muscle cells. Zhongguo Zu Zhi Gong Cheng Yan Jiu Yu Lin Chuang Kang Fu. 37:7258–7262. 2008.(In Chinese).
8	Li BL, Zhao XS, Luo R, et al: Influence of sub-health fatigue serum on protein expression of human skeletal muscle cells. Zhong guo Zu Zhi Gong Cheng Yan Jiu Yu Lin Chuang Kang Fu. 11:2095–2100. 2009.(In Chinese).
9	McCully KK, Smith S, Rajaei S, Leigh JS Jr and Natelson BH: Blood flow and muscle metabolism in chronic fatigue syndrome. Clin Sci (Lond). 104:641–647. 2003. View Article : Google Scholar : PubMed/NCBI
10	McCully KK, Smith S, Rajaei S, Leigh JS Jr and Natelson BH: Muscle metabolism with blood flow restriction in chronic fatigue syndrome. J Appl Physiol. 96:871–878. 2004. View Article : Google Scholar : PubMed/NCBI
11	McCully KK, Malucelli E and Iotti S: Increase of free Mg²⁺ in the skeletal muscle of chronic fatigue syndrome patients. Dyn Med. 5:12006. View Article : Google Scholar
12	Meeus M, Nijs J, McGregor N, et al: Unravelling intracellular immune dysfunctions in chronic fatigue syndrome: interactions between protein kinase R activity, RNase L cleavage and elastase activity, and their clinical relevance. In Vivo. 22:115–121. 2008.
13	Kerr JR, Petty R, Burke B, et al: Gene expression subtypes in patients with chronic fatigue syndrome/myalgic encephalomyelitis. J Infect Dis. 197:1171–1184. 2008. View Article : Google Scholar : PubMed/NCBI
14	Maes M, Mihaylova I, Kubera M and Bosmans E: Not in the mind but in the cell: increased production of cyclo-oxygenase-2 and inducible NO synthase in chronic fatigue syndrome. Neuro Endocrinol Lett. 28:463–469. 2007.PubMed/NCBI
15	Broderick G, Fuite J, Kreitz A, Vernon SD, Klimas N and Fletcher MA: A formal analysis of cytokine networks in chronic fatigue syndrome. Brain Behav Immun. 24:1209–1217. 2010. View Article : Google Scholar : PubMed/NCBI
16	Myhill S, Booth NE and McLaren-Howard J: Chronic fatigue syndrome and mitochondrial dysfunction. Int J Clin Med. 2:1–16. 2009.
17	Zhao XS, Luo R, Zhang ZQ, et al: Research on related genes of kidney yin deficiency syndrome. Zhonghua Xian Dai Yi Xue Za Zhi. 17:285–286. 2007.(In Chinese).
18	Dai FG, Zhao XS, Luo R, et al: Construction of DNA subtractive library of deficiency of kidney yin at sub-health state for Chinese Han people. Zhongguo Lin Chuang Kang Fu. 19:97–99. 2005.(In Chinese).
19	Sun XM, Li XY and Jin W: The initial study on plasma proteomics of sub-health deficiency of kidney-YIN. J Sichuan Trad Chin Med. 26:7–9. 2008.
20	White AT, Light AR, Hughen RW, Vanhaitsma TA and Light KC: Differences in metabolite-detecting, adrenergic, and immune gene expression after moderate exercise in patients with chronic fatigue syndrome, patients with multiple sclerosis, and healthy controls. Psychosom Med. 74:46–54. 2012. View Article : Google Scholar
21	Nguyen DV, Arpat AB, Wang N and Carroll RJ: DNA microarray experiments: biological and technological aspects. Biometrics. 58:701–717. 2002. View Article : Google Scholar : PubMed/NCBI
22	Xiang Z, Yang Y, Ma X and Ding W: Microarray expression profiling: analysis and applications. Curr Opin Drug Discov Devel. 6:384–395. 2003.PubMed/NCBI
23	Chen W, Wu ZY and Qiu JF: Gene expression profile in hepatocytes of rats undergoing hypoxia pre-conditioning. Zhonghua Wai Ke Za Zhi. 43:1405–1406. 2005.(In Chinese).
24	Shi XH, Sun L and Yang Z: Microarray detection of gene expression profiles difference of skeletal muscle between normal rats and those with single bout of exhaustive exercise. Zhong Guo Zu Zhi Gong Cheng Yan Jiu Yu Lin Chuang Kang Fu. 9:140–143. 2006.(In Chinese).
25	Whistler T, Taylor R, Craddock RC, Broderick G, Klimas N and Unger ER: Gene expression correlates of unexplained fatigue. Pharmacogenomics. 7:395–405. 2006. View Article : Google Scholar : PubMed/NCBI
26	Vernon SD, Unger ER, Dimulescu IM, Rajeevan M and Reeves WC: Utility of the blood for gene expression profiling and biomarker discovery in chronic fatigue syndrome. Dis Markers. 18:193–199. 2002. View Article : Google Scholar : PubMed/NCBI
27	Kaushik N, Fear D, Richards SC, et al: Gene expression in peripheral blood mononuclear cells from patients with chronic fatigue syndrome. J Clin Pathol. 58:826–832. 2005. View Article : Google Scholar : PubMed/NCBI
28	Smith AK, Fang H, Whistler T, Unger ER and Rajeevan MS: Convergent genomic studies identify association of GRIK2 and NPAS2 with chronic fatigue syndrome. Neuropsychobiology. 64:183–194. 2011. View Article : Google Scholar : PubMed/NCBI
29	Arya R, Mallik M and Lakhotia SC: Heat shook genes-integrating cell survival and death. J Biosci. 32:595–610. 2007. View Article : Google Scholar : PubMed/NCBI
30	Arrigo AP: Small stress proteins: chaperones that act as regulators of intracellular redox state and programmed cell death. Biol Chem. 379:19–26. 1998.PubMed/NCBI
31	Nelson RJ, Ziegelhoffer T, Nicolet C, Werner-Washburne M and Craig EA: The translation machinery and 70 kd heat shock protein cooperate in protein synthesis. Cell. 71:97–105. 1992. View Article : Google Scholar : PubMed/NCBI
32	Gething MJ and Sambrook J: Protein folding in the cell. Nature. 355:33–45. 1992. View Article : Google Scholar : PubMed/NCBI
33	Liu Y, Lormes W, Wang L, Reissnecker S and Steinacker JM: Different skeletal muscle HSP70 responses to high-intensity strength training and low-intensity endurance training. Eur J Appl Physiol. 91:330–335. 2004. View Article : Google Scholar : PubMed/NCBI
34	Naito H, Powers SK, Demirel HA and Aoki J: Exercise training increases heat shock protein in skeletal muscles of old rats. Med Sci Sports Exerc. 33:729–734. 2001. View Article : Google Scholar : PubMed/NCBI
35	Gao QJ, Ma XD and Xie LR: Research development of skeletal muscle cytoskeleton and small heat shock proteins. Chin Sport Sci. 25:70–74. 2005.