Dexamethasone inhibits TGF-β2-induced migration of human lens epithelial cells: Implications for posterior capsule opacification prevention

  • Authors:
    • Jin Yao
    • Wen Yang
    • Yuan Liu
    • Yan-Xi Sun
    • Qin Jiang
  • View Affiliations

  • Published online on: March 8, 2012     https://doi.org/10.3892/mmr.2012.827
  • Pages: 1509-1513
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Abstract

The elevation of transforming growth factor-β2 (TGF-β2) levels in eye tissue is considered as one of the major factors contributing to posterior capsule opacification (PCO) in patients undergoing cataract surgery, since TGF-β2 is known to stimulate the cell migration of residual human lens epithelial cells (HLECs). The present study aimed to test the potential effect of dexamethasone (DEX) on TGF-β2-induced cell migration and the possible cellular mechanisms involved in this process. Cultured HLE-B3 cells were treated with TGF-β2 (0.1 ng/ml) in the presence or absence of DEX (100 nM). HLE-B3 cell migration was determined by the Phagokinetic Track Motility Assay. Activation of mitogen-activated protein kinase (MAPK) signaling pathways was determined by Western blotting using specific phosphorylation antibodies, matrix metalloproteinase (MMP)-2 and MMP-9 mRNA expression, and activities were analyzed by RT-PCR and gelatin zymography assay, respectively. In cultured HLE-B3 cells, DEX largely inhibited TGF-β2-induced cell migration and MMP activity, probably by inhibiting the ERK/MAPK pathway. We suggest that the use of DEX may be of help in the prevention of PCO formation and development.

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June 2012
Volume 5 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Yao J, Yang W, Liu Y, Sun Y and Jiang Q: Dexamethasone inhibits TGF-β2-induced migration of human lens epithelial cells: Implications for posterior capsule opacification prevention. Mol Med Rep 5: 1509-1513, 2012.
APA
Yao, J., Yang, W., Liu, Y., Sun, Y., & Jiang, Q. (2012). Dexamethasone inhibits TGF-β2-induced migration of human lens epithelial cells: Implications for posterior capsule opacification prevention. Molecular Medicine Reports, 5, 1509-1513. https://doi.org/10.3892/mmr.2012.827
MLA
Yao, J., Yang, W., Liu, Y., Sun, Y., Jiang, Q."Dexamethasone inhibits TGF-β2-induced migration of human lens epithelial cells: Implications for posterior capsule opacification prevention". Molecular Medicine Reports 5.6 (2012): 1509-1513.
Chicago
Yao, J., Yang, W., Liu, Y., Sun, Y., Jiang, Q."Dexamethasone inhibits TGF-β2-induced migration of human lens epithelial cells: Implications for posterior capsule opacification prevention". Molecular Medicine Reports 5, no. 6 (2012): 1509-1513. https://doi.org/10.3892/mmr.2012.827