β-Nerve growth factor attenuates hepatocyte injury induced by D-galactosamine in vitro via TrkANGFR

  • Authors:
    • Jun-Feng Li
    • Jian-Chang Shu
    • Shao-Hui Tang
    • Yan-Mei Deng
    • Mei-Ya Fu
    • Xia Lv
  • View Affiliations

  • Published online on: July 16, 2013     https://doi.org/10.3892/mmr.2013.1590
  • Pages: 813-817
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Nerve growth factor (NGF) regulates the proliferation, differentiation and survival of cells and is also involved in the wound healing and tissue remodeling processes. The biological effects of NGF are dependent upon receptor signal-mediating functions, which differ between cells. This study attempted to investigate the hepatoprotective effect and possible mechanism of β-NGF on D-galactosamine (D-GalN)‑injured human liver L-02 cell lines. We demonstrated that L-02 cells expressed the neurotrophin receptors tyrosine kinase-A nerve growth factor receptor (TrkANGFR) and p75 pan-neurotrophin receptor (p75NTR). Recombinant human β-NGF markedly reduced cell injury and promoted the proliferation of L-02 cells damaged by D-GalN. However, this proliferation effect was blocked by the anti-TrkANGFR antibody. Lactate dehydrogenase (LDH) and malondialdehyde (MDA) were released at reduced levels in the L-02 cell culture supernatant pretreated with β-NGF. Furthermore, the albumin (ALB) content in the cell medium and intracellular glutathione (GSH) levels were markedly augmented, and the permeability of the mitochondrial membrane of the L-02 cells was improved by β-NGF. Our results suggested that exogenous β-NGF protects L-02 cells from D-GalN-induced injury through the NGF/TrkANGFR signaling pathway.
View Figures
View References

Related Articles

Journal Cover

September 2013
Volume 8 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Li J, Shu J, Tang S, Deng Y, Fu M and Lv X: β-Nerve growth factor attenuates hepatocyte injury induced by D-galactosamine in vitro via TrkANGFR. Mol Med Rep 8: 813-817, 2013
APA
Li, J., Shu, J., Tang, S., Deng, Y., Fu, M., & Lv, X. (2013). β-Nerve growth factor attenuates hepatocyte injury induced by D-galactosamine in vitro via TrkANGFR. Molecular Medicine Reports, 8, 813-817. https://doi.org/10.3892/mmr.2013.1590
MLA
Li, J., Shu, J., Tang, S., Deng, Y., Fu, M., Lv, X."β-Nerve growth factor attenuates hepatocyte injury induced by D-galactosamine in vitro via TrkANGFR". Molecular Medicine Reports 8.3 (2013): 813-817.
Chicago
Li, J., Shu, J., Tang, S., Deng, Y., Fu, M., Lv, X."β-Nerve growth factor attenuates hepatocyte injury induced by D-galactosamine in vitro via TrkANGFR". Molecular Medicine Reports 8, no. 3 (2013): 813-817. https://doi.org/10.3892/mmr.2013.1590