Detection of DNA hypermethylation in sera of patients with Crohn's disease

Bae,Jin-Han; Park,Jongha; Yang,Kwang Mo; Kim,Tae-Oh; Yi,Joo Mi

doi:10.3892/mmr.2013.1840

Detection of DNA hypermethylation in sera of patients with Crohn's disease

Authors:
- Jin-Han Bae
- Jongha Park
- Kwang Mo Yang
- Tae-Oh Kim
- Joo Mi Yi
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Affiliations: Research Center, Dongnam Institute of Radiological and Medical Sciences (DIRAMS), Busan, Republic of Korea, Department of Internal Medicine, Inje University Haeundae Paik Hospital, Busan, Republic of Korea
Published online on: December 3, 2013 https://doi.org/10.3892/mmr.2013.1840
Pages: 725-729

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Abstract

Mounting evidence suggests that inflammatory bowel disease (IBD) is caused by genetic predisposition of various genes as well as an abnormal interaction with environmental factors, resulting in epigenetic alterations. It has become evident that epigenetic factors play a significant contributory role during disease development. Additionally, DNA methylation has been reported to be correlated with the development of IBD. In the present study, we examined the role of DNA hypermethylation in Crohn's disease (CD) patients. The transcription elongation regulator 1-like (TCERG1L) gene, which has been previously reported to be highly frequently methylated in colon tumors was selected as a candidate for the early detection of biomarkers for colon cancer patients. DNA methylation of TCERG1L in 101 serum samples of CD patients was examined. Results of conventional MSP analysis revealed high methylation [57% (58/101)] of serum samples in CD patients. The DNA methylation pattern of TCEEG1L was confirmed using bisulfate sequencing analysis. The results of the present study suggest that using regular colonoscopic surveillance sensitive DNA methylation markers may detect serum samples of CD patients, leading to reduced risk or prevention of the progression of advanced stages of disease.

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