An integrated analysis of the effects of microRNA and mRNA on esophageal squamous cell carcinoma

Yang,Yong; Li,Dianbo; Yang,Yang; Jiang,Gening

doi:10.3892/mmr.2015.3557

An integrated analysis of the effects of microRNA and mRNA on esophageal squamous cell carcinoma

Authors:
- Yong Yang
- Dianbo Li
- Yang Yang
- Gening Jiang
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Affiliations: Department of Thoracic Surgery, Shanghai Pulmonary Hospital Affiliated Tongji University, Shanghai 200433, P.R. China, Department of Thoracic Surgery, Linyi Tumor Hospital, Linyi, Shandong 276001, P.R. China
Published online on: March 27, 2015 https://doi.org/10.3892/mmr.2015.3557
Pages: 945-952
Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Esophageal squamous cell cancer (ESCC) is an aggressive type of cancer with poor prognosis and leading to decreased quality of life. The identification of patients at increased risk of esophageal squamous cell cancer may improve current understanding of the role of micro (mi)RNA in tumorigenesis, since the miRNA pattern of these patients may be associated with tumorigenesis. In the present study, the miRNA and mRNA expression profiles of ESCC tissue samples and adjacent normal control tissue samples were obtained from two dependent GEO series. Bioinformatics analyses, including the use of the Gene Oncology and Kyoto Encyclopedia of Genes and Genomes databases, were used to identify genes and pathways, which were specifically associated with miRNA‑associated ESCC oncology. A total of 17 miRNAs and 1,670 probes were differentially expressed in the two groups, and the differentially expressed miRNA and target interactions were analyzed. The mRNA of miRNA target genes were found to be involve 49 GO terms and 14 pathways. Of the genes differentially expressed between the two groups, miRNA‑181a, miRNA‑202, miRNA‑155, FNDC3B, BNC2 and MBD2 were the most significantly altered and may be important in the regulatory network. In the present study, a novel pattern of differential miRNA‑target expression was constructed, which with further investigation, may provide novel targets for diagnosing and understanding the mechanism of ESCC.

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