Lentivirus‑mediated knockdown of MeCP2 inhibits the growth of colorectal cancer cells in vitro

Song,Ning; Li,Keqiang; Wang,Yan; Chen,Zongyou; Shi,Liubin

doi:10.3892/mmr.2015.4612

Lentivirus‑mediated knockdown of MeCP2 inhibits the growth of colorectal cancer cells in vitro

Authors:
- Ning Song
- Keqiang Li
- Yan Wang
- Zongyou Chen
- Liubin Shi
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Affiliations: General Surgery Department, The Affiliated Huashan Hospital of Fudan University, Shanghai 200040, P.R. China, Oncomolecular Biology Laboratory of Ningbo No. 2 Hospital, Ningbo, Zhejiang 315010, P.R. China
Published online on: November 24, 2015 https://doi.org/10.3892/mmr.2015.4612
Pages: 860-866

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Abstract

Methyl‑CpG‑binding protein 2 (MeCP2) is a transcriptional repressor that has been implicated in tumor onset and progression. Compared with normal and other tumorous tissue, MeCP2 is highly expressed in well‑differentiated adenocarcinoma and mucinous adenocarcinoma tissues, particularly at the invasion site of colorectal cancer tissues. The aim of the present study was to evaluate the potential of MeCP2 for use as a therapeutic target for human colorectal cancer. The DLD‑1 colorectal cancer cell line was subjected to lentivirus‑mediated short hairpin RNA‑induced knockdown of MeCP2 and the effects on cell growth, cell cycle progression and cell migration were assessed. It was confirmed that lentivirus‑mediated RNA interference successfully suppressed MeCP2 expression in vitro, which was demonstrated to result in reduced cell viability, cell cycle arrest in G0/G1 phase and inhibition of cell migration. These results indicated that MeCP2 may serve as a potential target for gene therapy of colorectal cancer.

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