Epigallocatechin-3-gallate suppresses alveolar epithelial cell apoptosis in seawater aspiration-induced acute lung injury via inhibiting STAT1-caspase-3/p21 associated pathway

Liu,Wei; Dong,Mingqing; Bo,Liyan; Li,Congcong; Liu,Qingqing; Li,Zhichao; Jin,Faguang

doi:10.3892/mmr.2015.4617

Epigallocatechin-3-gallate suppresses alveolar epithelial cell apoptosis in seawater aspiration-induced acute lung injury via inhibiting STAT1-caspase-3/p21 associated pathway

Authors:
- Wei Liu
- Mingqing Dong
- Liyan Bo
- Congcong Li
- Qingqing Liu
- Zhichao Li
- Faguang Jin
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Affiliations: Department of Pulmonary Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710038, P.R. China, Department of Pathology and Pathophysiology, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China
Published online on: November 27, 2015 https://doi.org/10.3892/mmr.2015.4617
Pages: 829-836

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Abstract

The apoptosis of alveolar epithelial cells is important in seawater aspiration‑induced acute lung injury (ALI). The present study aimed to investigate whether epigallocatechin-3-gallate (EGCG) is able to suppress apoptosis in alveolar epithelial cells in seawater aspiration‑induced ALI in vivo and in vitro, and the possible mechanisms underlying it. The results indicated that seawater aspiration‑induced ALI in rats is accompanied by increased apoptosis in lung tissue cells and the expression of apoptosis‑associated proteins, caspase‑3 and p21. EGCG pretreatment significantly ameliorated seawater aspiration‑induced ALI. Furthermore, EGCG decreased seawater aspiration‑induced apoptosis and the expression of caspase‑3 and p21 in lung tissue cells. Seawater‑challenged A549 cells experienced increased apoptosis and elevated levels of phosphorylated‑signal transducer and activator of transcription 1 (P‑STAT1). EGCG pretreatment of the cells resulted in significantly decreased seawater‑induced apoptosis and lower levels of STAT1 and P‑STAT1 in A549 cells. This suggests that EGCG suppresses alveolar epithelial cell apoptosis in seawater aspiration‑induced ALI via inhibiting the STAT1-caspase-3/p21 associated pathway.

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