Open Access

Effects of integrin α5β1 on the proliferation and migration of human aortic vascular smooth muscle cells

  • Authors:
    • Yan Song
    • Xiaoyu Qin
    • Hanjie Wang
    • Renying Miao
    • Yonggan Zhang
    • Chaofeng Miao
    • Zifan Wang
  • View Affiliations

  • Published online on: December 7, 2015     https://doi.org/10.3892/mmr.2015.4649
  • Pages: 1147-1155
  • Copyright: © Song et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Integrin (ITG) α5β1 is a dominant fibronectin receptor that is abundantly expressed on the surface of vascular smooth muscle cells (VSMCs). However, the association between integrin α5β1 and the proliferation and migration of VSMCs has yet to be elucidated. The aim of the present study was to characterize the roles of ITGα5 and ITGβ1 in the proliferation and migration of VSMCs, and to determine the effects of ITGα5β1 on integrin‑linked kinase (ILK) and focal adhesion kinase (FAK) mRNA expression. Lentiviral expression vectors as well as RNA interference vectors of ITGα5 and ITGβ1 were successfully constructed and transfected into VSMCs to obtain ITGα5‑ and ITGβ1‑overexpressing or ‑silenced cells, respectively. Cell cycle distribution, proliferation and migration were analyzed in the transfected VSMCs in order to clarify the roles of ITGβ1 and ITGα5 in the proliferation and migration of VSMCs. ITGβ1 was markedly associated with the proliferation and migration of VSMCs, and FAK was shown to be involved in the signaling pathways of ITGβ1. ITGα5 did not exert any effects on VSMCs. The results of the present study may provide a possible therapeutic target for the prevention and treatment of early vascular disease associated with VSMCs.
View Figures
View References

Related Articles

Journal Cover

February-2016
Volume 13 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Song Y, Qin X, Wang H, Miao R, Zhang Y, Miao C and Wang Z: Effects of integrin α5β1 on the proliferation and migration of human aortic vascular smooth muscle cells. Mol Med Rep 13: 1147-1155, 2016
APA
Song, Y., Qin, X., Wang, H., Miao, R., Zhang, Y., Miao, C., & Wang, Z. (2016). Effects of integrin α5β1 on the proliferation and migration of human aortic vascular smooth muscle cells. Molecular Medicine Reports, 13, 1147-1155. https://doi.org/10.3892/mmr.2015.4649
MLA
Song, Y., Qin, X., Wang, H., Miao, R., Zhang, Y., Miao, C., Wang, Z."Effects of integrin α5β1 on the proliferation and migration of human aortic vascular smooth muscle cells". Molecular Medicine Reports 13.2 (2016): 1147-1155.
Chicago
Song, Y., Qin, X., Wang, H., Miao, R., Zhang, Y., Miao, C., Wang, Z."Effects of integrin α5β1 on the proliferation and migration of human aortic vascular smooth muscle cells". Molecular Medicine Reports 13, no. 2 (2016): 1147-1155. https://doi.org/10.3892/mmr.2015.4649