Open Access

Liposome‑delivered baicalein induction of myeloid leukemia K562 cell death via reactive oxygen species generation

  • Authors:
    • Scarlet Xiaoyan Wang
    • Xuesong Wen
    • Celia Bell
    • Sandra Appiah
  • View Affiliations

  • Published online on: January 8, 2018     https://doi.org/10.3892/mmr.2018.8396
  • Pages: 4524-4530
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Baicalein (BL), a potential cancer chemopreventative flavone, has been reported to inhibit cancer cell growth by inducing apoptosis and causing cell cycle arrest in various human cancer cell models. Delivery of BL via nanoliposomes has been shown to improve its oral bioavailability and long‑circulating property in vivo. However, the role of BL in the inhibition of human chronic myeloid leukemia (CML) K562 cell growth and its underlying mechanisms has yet to be elucidated. In the present study, BL was formulated into liposomes with different sizes to improve its solubility and stability. The cytotoxic and pro‑apoptotic effects of free BL and liposomal BL were also evaluated. The results demonstrated that 100 nm liposomes were the most stable formulation when compared with 200 and 400 nm liposomes. Liposomal BL inhibited K562 cell growth as efficiently as free BL (prepared in DMSO), indicating that the liposome may be a potential vehicle to deliver BL for the treatment of CML. Flow cytometry analysis showed that there was significant (P<0.005) cell cycle arrest in the sub‑G1 phase (compared with vehicle control), indicating cell apoptosis following 20 µM liposomal BL or free BL treatment of K562 cells for 48 h. The induction of cell apoptosis by all BL preparations was further confirmed through the staining of treated cells with Annexin V‑fluorescein isothiocyanate/propidium iodide. A significant increase in reactive oxygen species (ROS) gene­ration was observed in free BL and liposomal BL treated cells, with a higher level of ROS produced from those treated with free BL. This indicated that cell apoptosis induced by BL may be via ROS generation and liposome delivery may further extend the effect through its long‑circulating property.
View Figures
View References

Related Articles

Journal Cover

March-2018
Volume 17 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Wang SX, Wen X, Bell C and Appiah S: Liposome‑delivered baicalein induction of myeloid leukemia K562 cell death via reactive oxygen species generation. Mol Med Rep 17: 4524-4530, 2018.
APA
Wang, S.X., Wen, X., Bell, C., & Appiah, S. (2018). Liposome‑delivered baicalein induction of myeloid leukemia K562 cell death via reactive oxygen species generation. Molecular Medicine Reports, 17, 4524-4530. https://doi.org/10.3892/mmr.2018.8396
MLA
Wang, S. X., Wen, X., Bell, C., Appiah, S."Liposome‑delivered baicalein induction of myeloid leukemia K562 cell death via reactive oxygen species generation". Molecular Medicine Reports 17.3 (2018): 4524-4530.
Chicago
Wang, S. X., Wen, X., Bell, C., Appiah, S."Liposome‑delivered baicalein induction of myeloid leukemia K562 cell death via reactive oxygen species generation". Molecular Medicine Reports 17, no. 3 (2018): 4524-4530. https://doi.org/10.3892/mmr.2018.8396