Downregulated GPD1 and MAGL protein levels as potential biomarkers for the metastasis of triple‑negative breast tumors to axillary lymph nodes

Simsek,Turgay; Bal Albayrak,Merve Gulsen; Akpinar,Gurler; Canturk,Nuh Zafer; Kasap,Murat

doi:10.3892/ol.2023.14167

Downregulated GPD1 and MAGL protein levels as potential biomarkers for the metastasis of triple‑negative breast tumors to axillary lymph nodes

Authors:
- Turgay Simsek
- Merve Gulsen Bal Albayrak
- Gurler Akpinar
- Nuh Zafer Canturk
- Murat Kasap
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Affiliations: Department of General Surgery, Kocaeli University Medical School, 41001 Kocaeli, Turkey, Department of Medical Biology, Kocaeli University Medical School, 41001 Kocaeli, Turkey
Published online on: November 23, 2023 https://doi.org/10.3892/ol.2023.14167
Article Number: 34

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Abstract

Glycerol‑3‑phosphate dehydrogenase (GPD1) and monoacylglycerol lipase (MAGL) levels are known to be significantly downregulated in both the tissue and serum samples of patients with triple‑negative breast cancer (TNBC), compared with other BC subtypes and healthy controls. As such, the association between GPD1 and MAGL levels and lymph node metastasis was evaluated in the present study. Utilizing western blotting, lymph node protein extracts from metastasized BC subtypes were analyzed and a significant downregulation of GPD1 and MAGL protein expression levels in the lymph node metastases was demonstrated in the TNBC subtype, compared with healthy controls. This finding further highlighted the potential use of these two proteins in early BC onset and metastasis detection.

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