Open Access

Liquid biopsy utilizing miRNA in patients with advanced breast cancer treated with cyclin‑dependent kinase 4/6 inhibitors

  • Authors:
    • Marcin Kubeczko
    • Patrycja Tudrej
    • Tomasz Tyszkiewicz
    • Aleksandra Krzywon
    • Małgorzata Oczko‑Wojciechowska
    • Michał Jarząb
  • View Affiliations

  • Published online on: February 28, 2024     https://doi.org/10.3892/ol.2024.14314
  • Article Number: 181
  • Copyright: © Kubeczko et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cyclin‑dependent kinase 4/6 inhibitors (CDK4/6is) are the mainstay of treatment of hormone receptor+/human epidermal growth factor receptor 2‑patients with advanced breast cancer (ABC). Despite improvements in overall survival, most patients experience disease progression. Biomarkers derived from a liquid biopsy are appealing for their potential to detect resistance to treatment earlier than computed tomography imaging. However, clinical data concerning microRNAs (miRNAs/miRs) in the context of CDK4/6is are lacking. Thus, the present study assessed the use of miRNAs in patients with ABC treated with CDK4/6is. Patients treated for ABC with CDK4/6is between June and August 2022 were eligible. miRNA expression analyses were performed using a TaqMan™ low‑density miRNA array. A total of 80 consecutive patients with ABC treated with CDK4/6is at Maria Sklodowska‑Curie National Research Institute of Oncology (Gliwice, Poland) were assessed, with 14 patients diagnosed with progressive disease at the time of sampling, 55 patients exhibited clinical benefit from CDK4/6i treatment and 11 patients were at the beginning of CDK4/6i treatment. Patients with disease progression had significantly higher levels of miR‑21 (P=0.027), miR‑34a (P=0.011), miR‑193b (P=0.032), miR‑200a (P=0.027) and miR‑200b (P=0.003) compared with patients who benefitted from CDK4/6i treatment. Significantly higher levels of miR‑34a expression were observed in patients with progressive disease than in patients beginning treatment (P=0.031). The present study demonstrated the potential innovative role of circulating miRNAs during CDK4/6i treatment. Plasma‑based expression of miR‑21, ‑34a, ‑193b, ‑200a and ‑200b effectively distinguished patients with ABC who responded to CDK4/6i treatment from patients who were resistant. However, longitudinal studies are required to verify the predictive and prognostic potential of miRNA.
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April-2024
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Spandidos Publications style
Kubeczko M, Tudrej P, Tyszkiewicz T, Krzywon A, Oczko‑Wojciechowska M and Jarząb M: Liquid biopsy utilizing miRNA in patients with advanced breast cancer treated with cyclin‑dependent kinase 4/6 inhibitors. Oncol Lett 27: 181, 2024
APA
Kubeczko, M., Tudrej, P., Tyszkiewicz, T., Krzywon, A., Oczko‑Wojciechowska, M., & Jarząb, M. (2024). Liquid biopsy utilizing miRNA in patients with advanced breast cancer treated with cyclin‑dependent kinase 4/6 inhibitors. Oncology Letters, 27, 181. https://doi.org/10.3892/ol.2024.14314
MLA
Kubeczko, M., Tudrej, P., Tyszkiewicz, T., Krzywon, A., Oczko‑Wojciechowska, M., Jarząb, M."Liquid biopsy utilizing miRNA in patients with advanced breast cancer treated with cyclin‑dependent kinase 4/6 inhibitors". Oncology Letters 27.4 (2024): 181.
Chicago
Kubeczko, M., Tudrej, P., Tyszkiewicz, T., Krzywon, A., Oczko‑Wojciechowska, M., Jarząb, M."Liquid biopsy utilizing miRNA in patients with advanced breast cancer treated with cyclin‑dependent kinase 4/6 inhibitors". Oncology Letters 27, no. 4 (2024): 181. https://doi.org/10.3892/ol.2024.14314