Molecular biomarkers, SNP genotyping
and rare mutation detection in cancer

8-9 October 2019
Mystras Grand Palace Resort, Sparta, Greece

Deadline for registrations: 30 September 2019


Title: Molecular biomarkers, SNP genotyping and rare mutation detection in cancer

2 DAYS Methods and strategies for molecular analyses in cancer

Target audience:
Researchers and clinicians at all levels interested in molecular methods for biomarker analyses of cancers.

Entrance qualifications:
Basic knowledge of molecular methods.

This course will present methods for nucleic acid analysis in cancer covering SNP genotyping, rare mutation analysis, methylation, copy number analysis, gene expression analysis, splice variants, microRNA and exosome analysis. The whole workflow from sampling, preanalytics, analytics and data analysis, and relevant guidelines are discussed. The course is lecture based.

The course contains:

  • Introduction to single nucleotide polymorphism, SNP
  • What is an SNP?
  • Methods for SNP analysis incl. qPCR, HRM and sequencing
  • Introduction to rare mutation detection, copy number variation and variance analyses
  • Methods for rare mutation analysis incl. CastPCR, RNase H dependent PCR, myT primers, Two-Tailed PCR, dPCR, SimSen Seq etc.
  • Introduction to epigenetic analyses
  • Methods for methylation analysis and other epigenetic variations incl. nucleosome modifications.
  • Introduction to gene expression analysis and splice variants as biomarkers
  • Methods for expression analysis incl. qPCR and sequencing
  • Introduction to microRNA and exosome analyses
  • Methods for microRNA analysis including microarrays, sequencing, qPCR and Two-Tailed PCR
  • New guidelines and regulations for molecular analyses including CEN, ISO and IVD-R
  • Quality control procedures in molecular analyses

The course is held by TATAA Biocenter, that has been a qPCR and NGS course provider since 2001 and have trained thousands of satisfied customers. We want to share our knowledge with you too!

Course leader
: Professor Mikael Kubista, TATAA Biocenter

Professor Kubista is the head of the Department of Gene Expression at the Institute of Biotechnology of the Czech Academy of Sciences, founder and CEO of the TATAA Biocenter (www.tataa.com) and founder of Life Genomics (www.lifegenomics.se). He was one of the pioneers contributing to the development of quantitative real-time PCR (qPCR) and introduced qPCR for single-cell expression profiling. He led the development of reagents for high-throughput single-cell expression profiling and quality control at TATAA. He also developed qPCR tomography for intracellular expression profiling. Kubista co-authored the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines and is member of the CEN and ISO workgroups drafting the guidelines on preanalytics and the consortium CANCER-ID, developing procedures for liquid biopsy analyses for the pharmaceutical industry. Most recently, TATAA Biocenter developed Two-Tailed PCR, which is currently the most sensitive and specific method for microRNA analysis.

For more information on courses, you can visit TATAA Biocenter website.

: Mystras Grand Palace, Sparta, Greece
Date: 8-9 October 2019
Time: 9:00 a.m. - 5:00 p.m.

Registration date-time: October 8, 2019; from 8:00 a.m. to 9:00 a.m.
Registration fees: 590 EUR (Academic delegates),  690 EUR (Industrial delegates)

For questions or any further requests please send an email to workshops@spandidos-publications.com.