Effect of aging on the tendon structure and tendon‑associated gene expression in mouse foot flexor tendon

  • Authors:
    • Yoichi Sugiyama
    • Kiyohito Naito
    • Kenji Goto
    • Yuko Kojima
    • Atsushi Furuhata
    • Mamoru Igarashi
    • Isao Nagaoka
    • Kazuo Kaneko
  • View Affiliations

  • Published online on: March 13, 2019     https://doi.org/10.3892/br.2019.1200
  • Pages: 238-244
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Abstract

To evaluate the biological changes in tendons during the aging process, the present study examined the effect of aging on the tendon structure, distribution of collagen types I and III, and expression of tendon‑associated genes, using flexor tendons in a mouse model. Histological assessment of the tendon structure and distribution of collagen types I and III were performed, and the expression of tendon‑associated genes was evaluated in flexor digitorium longus tendons of young (8 weeks) and aged (78 weeks) female C57BL/6 mice. The results indicated that the Soslowsky score, based on the analysis of cellularity, fibroblastic changes, and collagen fiber orientation and disruption, was significantly increased, or worsened, in the tendons of the aged group compared with those in the young group. Furthermore, in the aged group, the distribution of type I collagen was decreased and the distribution of type III collagen was relatively increased compared with the young group. Finally, the mRNA expression levels of collagen (type I and type III) and tenogenic markers (Mohawk homeobox, tenomodulin and scleraxis BHLH transcription factor) were significantly decreased in the aged group compared with the young group. The present observations demonstrated that the structure of the tendons, distribution of types I and III collagen and the expression of tendon‑associated genes were modulated by aging in the flexor tendon, and that these changes may contribute to the degeneration of tendons in tendinopathy.
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April-2019
Volume 10 Issue 4

Print ISSN: 2049-9434
Online ISSN:2049-9442

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Spandidos Publications style
Sugiyama Y, Naito K, Goto K, Kojima Y, Furuhata A, Igarashi M, Nagaoka I and Kaneko K: Effect of aging on the tendon structure and tendon‑associated gene expression in mouse foot flexor tendon. Biomed Rep 10: 238-244, 2019.
APA
Sugiyama, Y., Naito, K., Goto, K., Kojima, Y., Furuhata, A., Igarashi, M. ... Kaneko, K. (2019). Effect of aging on the tendon structure and tendon‑associated gene expression in mouse foot flexor tendon. Biomedical Reports, 10, 238-244. https://doi.org/10.3892/br.2019.1200
MLA
Sugiyama, Y., Naito, K., Goto, K., Kojima, Y., Furuhata, A., Igarashi, M., Nagaoka, I., Kaneko, K."Effect of aging on the tendon structure and tendon‑associated gene expression in mouse foot flexor tendon". Biomedical Reports 10.4 (2019): 238-244.
Chicago
Sugiyama, Y., Naito, K., Goto, K., Kojima, Y., Furuhata, A., Igarashi, M., Nagaoka, I., Kaneko, K."Effect of aging on the tendon structure and tendon‑associated gene expression in mouse foot flexor tendon". Biomedical Reports 10, no. 4 (2019): 238-244. https://doi.org/10.3892/br.2019.1200