Evaluation of non‑coding region sequence variants and mitochondrial haplogroups as potential biomarkers of sporadic breast cancer in individuals of Sri Lankan Sinhalese ethnicity

  • Authors:
    • Joanne Tiphania Kotelawala
    • Ruwandi Ranasinghe
    • Chrishani Rodrigo
    • Kamani Hemamala Tennekoon
    • Kanishka De Silva
  • View Affiliations

  • Published online on: March 17, 2020     https://doi.org/10.3892/br.2020.1292
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Abstract

Mitochondrial DNA (mtDNA) mutations have been reported to be associated with various diseases, including cancer. The present study investigated the mtDNA non‑coding region mutations and mitochondrial haplogroups as potential biomarkers of sporadic breast cancer in Sri Lankan Sinhalese women. Mitochondrial macro‑haplogroups were determined using PCR‑restriction fragment length polymorphism, whereas non‑coding region sequences were determined using Sanger sequencing. The sequence of the non‑coding region was also used to confirm haplogroup status. Neither the mutations in the non‑coding region nor the mitochondrial haplogroups that were reported as risk factors in other populations, were determined to be potential risk factors for sporadic breast cancer in the present study. Furthermore, several novel mutations were identified in the present matched pairs case‑controlled study. The M65a haplogroup with an additional mutation at position 16311 (P=0.0771) and mutations at the ori‑b site (P=0.05) were considered a weak risk factor and protective factor, respectively, for sporadic breast cancer in Sinhalese women. Previous studies have indicated the use of mtDNA mutations as a biomarker; however, the present study showed that such biomarkers need to be validated for individual ethnic groups before they can be recommended for use in the prediction of disease.

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APA
Kotelawala, J.T., Ranasinghe, R., Rodrigo, C., Tennekoon, K.H., & De Silva, K. (1899). Evaluation of non‑coding region sequence variants and mitochondrial haplogroups as potential biomarkers of sporadic breast cancer in individuals of Sri Lankan Sinhalese ethnicity. Biomedical Reports, 0, 0-0. https://doi.org/10.3892/br.2020.1292
MLA
Kotelawala, J. T., Ranasinghe, R., Rodrigo, C., Tennekoon, K. H., De Silva, K."Evaluation of non‑coding region sequence variants and mitochondrial haplogroups as potential biomarkers of sporadic breast cancer in individuals of Sri Lankan Sinhalese ethnicity". Biomedical Reports 0.0 (1899): 0-0.
Chicago
Kotelawala, J. T., Ranasinghe, R., Rodrigo, C., Tennekoon, K. H., De Silva, K."Evaluation of non‑coding region sequence variants and mitochondrial haplogroups as potential biomarkers of sporadic breast cancer in individuals of Sri Lankan Sinhalese ethnicity". Biomedical Reports 0, no. 0 (1899): 0-0. https://doi.org/10.3892/br.2020.1292