Open Access

Matrigel scaffold combined with Ad-hBMP7-transfected chondrocytes improves the repair of rabbit cartilage defect

  • Authors:
    • Xiaopeng Xia
    • Jing Li
    • Bo Xia
    • Huilin Yang
    • Dongmei Zhang
    • Bin Zhou
    • Jie Zhang
    • Man Zhou
    • Fan Liu
  • View Affiliations

  • Published online on: December 13, 2016     https://doi.org/10.3892/etm.2016.3972
  • Pages: 542-550
  • Copyright: © Xia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: HTML 0 views | PDF 0 views     Cited By (CrossRef): 0 citations

Abstract

The aim of this study was to explore an effective method for the repair of cartilage defects using chitosan/glycerophosphate (C/GP) gel- and Matrigel-engineered human bone morphogenetic protein 7 (hBMP7)-expressing chondrocytes. Rabbit chondrocytes were obtained, cultured in vitro and transfected with an adenovirus containing hBMP7 and green fluorescent protein (Ad‑hBMP7‑GFP). The expression of hBMP7 in the transfected cells was tested by reverse transcription-polymerase chain reaction (RT‑PCR) and western blotting. The phenotype of the transfected cells was evaluated by detecting the yields of collagen II and hyaluronic acid using RT‑PCR and enzyme‑linked immunosorbent assay (ELISA). The growth of chondrocytes in the C/GP gel and Matrigel was accessed by measuring the cell growth rate, hematoxylin and eosin (H&E) staining and observation under a scanning microscope. Twelve adult male New Zealand white rabbits were randomly divided into three groups. Two cartilage defects were created in the rabbits' knees by aseptic surgery. Group A (n=4) did not receive any treatment, group B (n=4) were treated with C/GP gel and Matrigel-engineered Ad‑mock‑GFP‑transfected chondrocytes, and group C (n=4) were treated with C/GP gel and Matrigel‑engineered Ad-hBMP7-GFP-transfected chondrocytes. Rabbits were sacrificed at 4 weeks after transplantation, and the repair effect was measured by the Wakitani scoring method. On the basis of the RT‑PCR and western blot results, hBMP7 was efficiently overexpressed in the Ad‑hBMP7‑GFP‑transfected chondrocytes. The ELISA results showed that the yields of collagen II and hyaluronic acid in Ad‑hBMP7‑GFP‑transfected chondrocytes were significantly higher than those in Ad‑mock‑GFP‑transfected chondrocytes. Chondrocytes have a better morphology and arrangement in a Matrigel scaffold than in C/GP, as assessed by H&E staining and scanning microscopy. According to the Wakitani score, Matrigel combined with Ad‑hBMP7‑GFP‑transfected chondrocytes successfully promoted the repair of cartilage defects in rabbit knees.

Related Articles

Journal Cover

February 2017
Volume 13 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Xia, X., Li, J., Xia, B., Yang, H., Zhang, D., Zhou, B. ... Liu, F. (2017). Matrigel scaffold combined with Ad-hBMP7-transfected chondrocytes improves the repair of rabbit cartilage defect. Experimental and Therapeutic Medicine, 13, 542-550. https://doi.org/10.3892/etm.2016.3972
MLA
Xia, X., Li, J., Xia, B., Yang, H., Zhang, D., Zhou, B., Zhang, J., Zhou, M., Liu, F."Matrigel scaffold combined with Ad-hBMP7-transfected chondrocytes improves the repair of rabbit cartilage defect". Experimental and Therapeutic Medicine 13.2 (2017): 542-550.
Chicago
Xia, X., Li, J., Xia, B., Yang, H., Zhang, D., Zhou, B., Zhang, J., Zhou, M., Liu, F."Matrigel scaffold combined with Ad-hBMP7-transfected chondrocytes improves the repair of rabbit cartilage defect". Experimental and Therapeutic Medicine 13, no. 2 (2017): 542-550. https://doi.org/10.3892/etm.2016.3972