Combination therapy with catechins and caffeine inhibits fat accumulation in 3T3-L1 cells

  • Authors:
    • Xiaojuan Zhu
    • Licong Yang
    • Feng Xu
    • Lezhen Lin
    • Guodong Zheng
  • View Affiliations

  • Published online on: December 15, 2016     https://doi.org/10.3892/etm.2016.3975
  • Pages: 688-694
Metrics: HTML 0 views | PDF 0 views     Cited By (CrossRef): 0 citations

Abstract

Catechins and caffeine, which are green tea components, have a slimming effect; however, the combinational effect of fat metabolism in 3T3‑L1 cells remains unclear. In the present study, 3T3‑L1 cells were treated with catechins and caffeine in combination, and it was found that combination therapy with catechins and caffeine markedly reduced intracellular fat accumulation, mRNA expression levels of peroxisome proliferator‑activated receptor‑γ and CCAAT/enhancer-binding protein α in the early stage of cell differentiation were significantly reduced, and mRNA expression of fatty acid synthetase (FAS) and glycerol‑3‑phosphate dehydrogenase protein expression levels of FAS were downregulated. Noradrenaline‑induced lipolysis was enhanced by caffeine, which markedly increased the protein expression of adipose triglyceride lipase and hormone sensitive lipase. These results indicated that combination therapy with catechins and caffeine synergistically inhibited lipid accumulation by regulating the gene and protein expression levels of lipid metabolism‑related enzymes. Therefore, catechins and caffeine combination therapy has potential as a functional food that may be used to prevent obesity and lifestyle-associated diseases.

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February 2017
Volume 13 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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APA
Zhu, X., Yang, L., Xu, F., Lin, L., & Zheng, G. (2017). Combination therapy with catechins and caffeine inhibits fat accumulation in 3T3-L1 cells. Experimental and Therapeutic Medicine, 13, 688-694. https://doi.org/10.3892/etm.2016.3975
MLA
Zhu, X., Yang, L., Xu, F., Lin, L., Zheng, G."Combination therapy with catechins and caffeine inhibits fat accumulation in 3T3-L1 cells". Experimental and Therapeutic Medicine 13.2 (2017): 688-694.
Chicago
Zhu, X., Yang, L., Xu, F., Lin, L., Zheng, G."Combination therapy with catechins and caffeine inhibits fat accumulation in 3T3-L1 cells". Experimental and Therapeutic Medicine 13, no. 2 (2017): 688-694. https://doi.org/10.3892/etm.2016.3975