Open Access

GFAP expression in injured astrocytes in rats

  • Authors:
    • Shuquan Zhang
    • Minfei Wu
    • Chuangang Peng
    • Guanjie Zhao
    • Rui Gu
  • View Affiliations

  • Published online on: July 10, 2017     https://doi.org/10.3892/etm.2017.4760
  • Pages: 1905-1908
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Glial fibrillary acidic protein (GFAP) is one of the best markers for the activation of astrocytes (AS) following injury or stress in the central nervous system (CNS). The purpose of this study was to examine the expression of GFAP and 14-3-3ε in rat AS subjected to hypoxia. We established primary cultures of AS from cerebral cortex of neonatal Sprague-Dawley rats as a model of glucose deficiency and hypoxia/ischemia-reperfusion. We analyzed the activated astrocyte markers GFAP and 14-3-3ε by western blot analysis and found that both increased over time, starting at 4 h and reaching the highest level at 72 h, at the end of the experiment. GFAP and 14-3-3ε protein localization by double‑labeling immunofluorescence showed elevated expression and co-localization in the cytoplasm of AS. GFAP and 14-3-3ε expression remained elevated in AS 72 h after stress conditions, which is possibly related to the excessive activation and dysfunction of the CNS in chronic injuries.

Related Articles

Journal Cover

September-2017
Volume 14 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhang S, Wu M, Peng C, Zhao G and Gu R: GFAP expression in injured astrocytes in rats. Exp Ther Med 14: 1905-1908, 2017
APA
Zhang, S., Wu, M., Peng, C., Zhao, G., & Gu, R. (2017). GFAP expression in injured astrocytes in rats. Experimental and Therapeutic Medicine, 14, 1905-1908. https://doi.org/10.3892/etm.2017.4760
MLA
Zhang, S., Wu, M., Peng, C., Zhao, G., Gu, R."GFAP expression in injured astrocytes in rats". Experimental and Therapeutic Medicine 14.3 (2017): 1905-1908.
Chicago
Zhang, S., Wu, M., Peng, C., Zhao, G., Gu, R."GFAP expression in injured astrocytes in rats". Experimental and Therapeutic Medicine 14, no. 3 (2017): 1905-1908. https://doi.org/10.3892/etm.2017.4760