MicroRNA‑320 regulates autophagy in retinoblastoma by targeting hypoxia inducible factor‑1α

  • Authors:
    • Yong Liang
    • Xi Chen
    • Zhu Liang
  • View Affiliations

  • Published online on: July 11, 2017     https://doi.org/10.3892/etm.2017.4779
  • Pages: 2367-2372
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Retinoblastoma (RB) is the most common malignancy in children. Due to refractory mechanisms of chemoresistance and the toxicity of chemotherapies, novel therapies for RB treatment are urgently required. MicroRNA‑320 (miR‑320) is believed to be associated with the tumorigenesis of RB, although the mechanism remains unclear. Considering the hypoxic intratumoral region, the roles of miR‑320 and hypoxia inducible factor‑1α (HIF‑1α) in the regulation of autophagy were investigated in 30 human RB samples and WERI‑RB1 cells. The results demonstrated that HIF‑1α was the downstream target of miR‑320, and decreased miRNA‑320 or HIF‑1α lead to the inhibition of autophagy in WERI‑RB1 cells. Compared with WERI‑RB1 cells that were not transfected, silenced HIF‑1α caused a 1.41‑fold increase (P<0.01) in p62, a 2.71‑fold decrease of Beclin1, and inhibited miRNA‑320. Silenced HIF‑1α also resulted in 7.29‑ and 7.43‑fold increases in phosphorylated‑mechanistic target of rapamycin (mTOR) and mTOR, respectively. In conclusion, the present results suggest that miRNA‑320 may regulate the development of autophagy by targeting HIF‑1α and autophagy‑related proteins in RB under hypoxic conditions.

Related Articles

Journal Cover

September 2017
Volume 14 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
APA
Liang, Y., Chen, X., & Liang, Z. (2017). MicroRNA‑320 regulates autophagy in retinoblastoma by targeting hypoxia inducible factor‑1α. Experimental and Therapeutic Medicine, 14, 2367-2372. https://doi.org/10.3892/etm.2017.4779
MLA
Liang, Y., Chen, X., Liang, Z."MicroRNA‑320 regulates autophagy in retinoblastoma by targeting hypoxia inducible factor‑1α". Experimental and Therapeutic Medicine 14.3 (2017): 2367-2372.
Chicago
Liang, Y., Chen, X., Liang, Z."MicroRNA‑320 regulates autophagy in retinoblastoma by targeting hypoxia inducible factor‑1α". Experimental and Therapeutic Medicine 14, no. 3 (2017): 2367-2372. https://doi.org/10.3892/etm.2017.4779