Glucagon-like peptide-1 receptor agonist exendin-4 protects against interleukin-1β-mediated inhibition of glucose-stimulated insulin secretion by mouse insulinoma β cells

  • Authors:
    • Ben Niu
    • Chao Li
    • Heng Su
    • Qingzhu Li
    • Qiu He
    • Lijuan Liu
    • Yuanming Xue
    • Tao Shen
    • Xueshan Xia
  • View Affiliations

  • Published online on: July 19, 2017     https://doi.org/10.3892/etm.2017.4803
  • Pages: 2671-2676
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Abstract

The aim of the present study was to investigate the protective effect of the glucagon‑like peptide‑1 receptor agonist exendin‑4 on the interleukin (IL)‑1β‑induced impairment of glucose‑stimulated insulin secretion (GSIS) in β‑TC‑6 cells. β‑TC‑6 cells were pretreated with various concentrations of IL‑1β (0.15, 1.5 or 15 ng/ml) and exendin‑4 (0.1 or 1 mM). Exendin‑4 was administered to β‑TC‑6 cells prior to, during and following pretreatment. Cells were stimulated with various concentrations of glucose (0, 1.38, 5.5 and 11.1 mM), and insulin was measured via radioimmunoassay of the supernatant; furthermore, western blot analysis was used to detect phosphorylated extracellular receptor kinase (ERK)1/2. The insulin levels (151.08±14.34 µIU/ml) and ERK1/2 phosphorylation in β‑TC‑6 cells peaked in response to 1.38 mM glucose stimulation compared with 0, 5.5 and 11.1 mM glucose stimulation. IL‑1β inhibited GSIS in a dose‑dependent manner: Insulin levels were 83.76±1.16 µIU/ml when 0.15 ng/ml IL‑1β was added under GSIS, 59.46±3.20 µIU/ml when 1.5 ng/ml IL‑1β was added under GSIS, and 56.98±1.19 µIU/ml when 15 ng/ml IL‑1β was added under GSIS. Exendin‑4 exerted a protective effect against IL‑1β‑induced GSIS inhibition in a dose‑dependent manner. The greatest protective effect was observed when exendin‑4 was added prior to IL‑1β pretreatment, which was statistically significant (P<0.05). These findings suggested that exendin‑4 was able to reverse the IL‑1β‑induced inhibition of ERK1/2 phosphorylation and serves a protective role by impairing GSIS induced by IL‑1β in β‑TC‑6 cells. This mechanism may be associated with the recovery of ERK1/2 activation.

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September-2017
Volume 14 Issue 3

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Spandidos Publications style
Niu B, Li C, Su H, Li Q, He Q, Liu L, Xue Y, Shen T and Xia X: Glucagon-like peptide-1 receptor agonist exendin-4 protects against interleukin-1β-mediated inhibition of glucose-stimulated insulin secretion by mouse insulinoma β cells. Exp Ther Med 14: 2671-2676, 2017.
APA
Niu, B., Li, C., Su, H., Li, Q., He, Q., Liu, L. ... Xia, X. (2017). Glucagon-like peptide-1 receptor agonist exendin-4 protects against interleukin-1β-mediated inhibition of glucose-stimulated insulin secretion by mouse insulinoma β cells. Experimental and Therapeutic Medicine, 14, 2671-2676. https://doi.org/10.3892/etm.2017.4803
MLA
Niu, B., Li, C., Su, H., Li, Q., He, Q., Liu, L., Xue, Y., Shen, T., Xia, X."Glucagon-like peptide-1 receptor agonist exendin-4 protects against interleukin-1β-mediated inhibition of glucose-stimulated insulin secretion by mouse insulinoma β cells". Experimental and Therapeutic Medicine 14.3 (2017): 2671-2676.
Chicago
Niu, B., Li, C., Su, H., Li, Q., He, Q., Liu, L., Xue, Y., Shen, T., Xia, X."Glucagon-like peptide-1 receptor agonist exendin-4 protects against interleukin-1β-mediated inhibition of glucose-stimulated insulin secretion by mouse insulinoma β cells". Experimental and Therapeutic Medicine 14, no. 3 (2017): 2671-2676. https://doi.org/10.3892/etm.2017.4803