Open Access

Human umbilical cord MSC‑derived hepatocyte growth factor enhances autophagy in AOPP‑treated HK‑2 cells

  • Authors:
    • Minhui Li
    • Tingting Jiang
    • Wenying Zhang
    • Wei Xie
    • Tingting Guo
    • Xun Tang
    • Jun Zhang
  • View Affiliations

  • Published online on: July 13, 2020     https://doi.org/10.3892/etm.2020.8998
  • Pages: 2765-2773
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Mesenchymal stem cell (MSC) transplantation may serve as an important treatment modality in chronic kidney disease (CKD); however, the underlying mechanisms remain unclear. Advanced oxidation protein products (AOPP) have been demonstrated to induce renal tubular epithelial cell (RTEC) injury via autophagy inhibition. Therefore, the present study was performed to investigate the role of human umbilical cord‑derived MSCs (hUC‑MSCs) in RTEC autophagy. AOPP‑treated HK‑2 cells were co‑cultured with hUC‑MSCs or treated with recombinant humanized hepatocyte growth factor (HGF). Western blotting was used to detect the levels of autophagy‑and PI3K/AKT/mTOR signaling pathway‑related proteins, and immunofluorescence staining was used to detect the levels of autophagy‑related proteins. The HGF protein levels in HK‑2 cells and the hUC‑MSC co‑culture system were measured. The cells were subsequently treated with tivantinib, an HGF competitive inhibitor, and the levels of autophagy‑related proteins were detected. Microtubule‑associated protein 1 light chain 3B (LC3B) II/LC3B I (LC3II/LC3I) and beclin 1 protein levels were increased, while p62, PI3K, phosphorylated (p)‑AKT and the p‑mTOR protein levels were decreased in AOPP‑treated HK‑2 cells co‑cultured with hUC‑MSC, compared with the group treated with AOPP only. Furthermore, HGF expression was increased in AOPP‑treated HK‑2 cells co‑cultured with hUC‑MSC, compared with the group treated with AOPP alone. When HGF activity was inhibited using tivantinib, these effects on LC3II/LC3I, beclin 1, p62, PI3K, p‑AKT, and p‑mTOR expression were partially reversed. Furthermore, the effects of tivantinib were reversed by Ly294002. In conclusion, the present study revealed that hUC‑MSCs partially reversed AOPP‑mediated inhibition of autophagy in HK‑2 cells via secretion of HGF, indicating that hUC‑MSCs may serve as a potential therapy for preventing the progression of CKD.

Related Articles

Journal Cover

September-2020
Volume 20 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Li M, Jiang T, Zhang W, Xie W, Guo T, Tang X and Zhang J: Human umbilical cord MSC‑derived hepatocyte growth factor enhances autophagy in AOPP‑treated HK‑2 cells. Exp Ther Med 20: 2765-2773, 2020
APA
Li, M., Jiang, T., Zhang, W., Xie, W., Guo, T., Tang, X., & Zhang, J. (2020). Human umbilical cord MSC‑derived hepatocyte growth factor enhances autophagy in AOPP‑treated HK‑2 cells. Experimental and Therapeutic Medicine, 20, 2765-2773. https://doi.org/10.3892/etm.2020.8998
MLA
Li, M., Jiang, T., Zhang, W., Xie, W., Guo, T., Tang, X., Zhang, J."Human umbilical cord MSC‑derived hepatocyte growth factor enhances autophagy in AOPP‑treated HK‑2 cells". Experimental and Therapeutic Medicine 20.3 (2020): 2765-2773.
Chicago
Li, M., Jiang, T., Zhang, W., Xie, W., Guo, T., Tang, X., Zhang, J."Human umbilical cord MSC‑derived hepatocyte growth factor enhances autophagy in AOPP‑treated HK‑2 cells". Experimental and Therapeutic Medicine 20, no. 3 (2020): 2765-2773. https://doi.org/10.3892/etm.2020.8998