Increased SPC24 in prostatic diseases and diagnostic value of SPC24 and its interacting partners in prostate cancer
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- Published online on: June 30, 2021 https://doi.org/10.3892/etm.2021.10355
- Article Number: 923
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Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Abstract
Introduction
SPC24 is a component of the nuclear division cycle 80 (NDC80) kinetochore complex and is involved in the formation of the kinetochore complex along with three other proteins, NUF2, NDC80 and SPC25(1). The NDC80 kinetochore complex mediates microtubule binding and is anchored onto the inner kinetochore via the SPC24-SPC25 protein complex. In addition, the SPC24-SPC25 protein complex mediates dynamic interactions between nuclear spindle microtubules and kinetochores, which ensures faithful and accurate chromosomal segregation during mitosis (2,3). Abnormal mitosis is a common hallmark of cancer (4). It has also been reported that mutation of SPC24 may lead to a chromosome segregation defect (5). Moreover, simultaneous disruption of both SPC24 and SPC25 may cause a spindle checkpoint defect in the cell, allowing the cell to bypass mitosis (6). These findings suggest that dysregulation of SPC24 may lead to genomic instability and disrupt control of the cell cycle, which can ultimately promote malignancy.
Prostate cancer (PCa) has been identified as the second most common cause of malignant tumor driven death in men (7). Dietary factors, lifestyle-related factors, region and genetic polymorphism have been recognized as contributors to the risk of PCa (8,9). Specific risk factors, such as prostatitis (inflammation/swelling of the prostate gland) and benign prostatic hypertrophy (BPH; proliferation of the cellular elements of the prostate, leading to an enlarged prostate gland) have been reported to have a strong association with the development of prostate cancer and a higher relative risk of prostate cancer was estimated in men with both of these conditions (10). BPH has shown a high correlation with prostate cancer in Asian populations (11). There is a 30% probability of biochemical relapse [prostate specific antigen (PSA) <0.2 ng/ml twice in a row with no recurrent or metastatic lesions found on radiography] in prostate cancer patients within the first 5 years after radical prostatectomy (12). In the clinic, detection of PSA is often used for early diagnosis of prostate cancer. However, PSA is not specific to malignant PCa and has a high false-positive and false-negative rate of ~15% (13). Therefore, a better PCa biomarkerwould be beneficial for accurate diagnosis.
High levels of SPC24 have been found to be expressed in various cancers, including breast, lung, thyroid and liver cancer and osteosarcoma (14-17). A recent study also indicated that increased SPC24 expression is associated with advanced stage lung tumors (15). An additional study indicated that when endogenous SPC24 was knocked down, the growth and invasion ability of anaplastic thyroid cancer cells was inhibited and cell apoptosis was promoted (16). However, the role played by SPC24 in PCa is unclear.
In the present study, RNA-Seq data from The Cancer Genome Atlas (TCGA) database were used to analyze SPC24 messenger RNA (mRNA) levels in PCa tissues and normal tissues. Subsequently, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot analysis were conducted to measure SPC24 expression in PCa and adjacent tissues, along with BPH tissues. SPC24 expression was also detected in 40 clinical BPH tissues, 35 PCa tissues, 35 paired adjacent tissues, 12 prostatitis tissues and 9 normal prostate tissues by immunohistochemistry (IHC). In these tissues, the association of SPC24 with clinicopathological features was also assessed. Gene ontology (GO) analyses, Reactome pathway analyses and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the identified genes were conducted to find correlations between SPC24 and its interactors in biological processes, which were confirmed by qPCR.
Materials and methods
Data collection and analysis of TCGA database
RNA sequencing (RNA-seq) data from selected genes (SPC24 and its interacting partners obtained from literature review and online database prediction) in PCa and clinicopathological information of patients were downloaded from the TCGA Prostate Cancer (PRAD) database (https://xena.ucsc.edu/public/; version 2017-10-13). This dataset indicates the gene-level transcription estimates, as log2(x+1) transformed RSEM (RNA-Seq by expectation-maximization) normalized counts. The 3rd level data (normalized and quantified raw data) was obtained from the TCGA data coordination center (cancer.gov/about-nci/organization/ccg/research/structural-genomics/tcga).
Patients and specimens
From 2016 to 2018, a total of 96 patients (age, 46-87 years) including 35 patients with PCa (median age, 65 years), 40 patients with BPH (median age, 64 years), 12 patients with prostatitis (median ages, 68 years) and 9 patients without prostate disease (median age, 62 years) participated in the present study. Written informed consent from patients and permission from the Ethics Committee of The First Affiliated Hospital of Guangxi Medical University were acquired. No preoperative treatment was performed before surgery. All samples were provided by the First Affiliated Hospital of the Guangxi Medical University. BPH tissues were collected from patients with transurethral plasmakinetic resection of prostate. PCa tissues and adjacent tissues were obtained from patients that underwent radical prostatectomy, while prostatitis tissues and normal tissues were obtained from patients that underwentradical cystectomy. All prostate samples with hematoxylin and eosin staining (H&E) were diagnosed by two expert pathologists. Tumor grades were calculated in accordance with Gleason classification. Clinical features of patients including ethnicity, age, smoking, drinking, PSA, urea, creatinine, lithic acid, HCO3-, and creatinine clearance (CrCl) were obtained by Medical History Taking, PSA Test, Blood Gas Test, and Renal Panel.
Quantitative real-time polymerase chain reaction
TRIzol® reagent (Invitrogen; Thermo Fisher Scientific, Inc.) was used to extract total RNA from the clinical BPH, PCa and adjacent tissues. A NanoDrop spectrophotometer (Thermo Fisher Scientific, Inc.) was used to assess the purity and quantity of the isolated RNA. Complementary DNA (cDNA) was synthesized using the Applied Biosystems StepOne (Thermo Fisher Scientific, Inc.) with qPCR kit and SYBR-Green master mix (Roche Diagnostics.). According to the instructions of the RT kit (PrimeScript™ RT Master Mix; Takara), RT was performed as follows: Initiation at 37˚C for 15 min, then 85˚C for 5 sec. The qPCR conditions included an initiation at 95˚C for 10 min, followed by 40 cycles of 95˚C for 15 sec and 60˚C for 1 min. The 2-ΔΔCq method (18) was performed to analyze relative quantities for the level of mRNA expression. The specific primer sequences were as follows: 5'-TGGCCTCAGCTAGGTAACCA-3' (forward) and 5'-GTACCTGGGAGCTGTCATCG-3' (reverse) for SPC24. 5'-GAAGCGCAGTTCAGTTTCC-3' (forward) and 5'-GGTTTCTCTTTGGTTTGAGGG-3' (reverse) for NDC80. 5'-TGGGAAAGATACATACAGTGG-3' (forward) and 5'-GAATCTTGGGTCATTGTGGT-3' (reverse) for BUB1.
Western blot analysis
Clinical PCa tissues and paired adjacent tissues were obtained, washed twice with 0.01 M PBS and lysed on ice with radioimmunoprecipitation assay (RIPA, Beijing Solarbio Science & Technology Co., Ltd.) a cell lysis reagent for 30 min. Tissues were centrifuged at 16,000 x g for 15 min at 4˚C to remove insoluble proteins. The concentration of extracted protein was determined by bicinchoninic acid (BCA) protein assay kit (Leagene, Inc.). A total of 50 µg of each total protein extract was separated using 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto PVDF membranes (Bio-Rad Laboratories, Inc.). After being blocked at 23˚C for 1 h with 5% milk solution, membranes were washed three times with Tris-buffered saline-tween (0.1% Tween-20) solution. The membranes were then incubated with primary antibodies, rabbit anti-human SPC24 (Abcam; 1:1,000; cat. no. ab169786) and β-actin (Proteintech Group, Inc.; 1:1,000; cat. no. HRP-60008) at 4˚C overnight. After being washed with TBST, the membranes were incubated with horseradish peroxidase-labeled goat-anti-rabbit IgG secondary antibody (Abcam; 1:4,000; cat. no. ab6721) at 37˚C for 1 h. The membranes were processed for protein detection using the BeyoECL Plus Kit (Beyotime Institute of Biotechnology).
Immunohistochemistry
All tissues were embedded in paraffin and then cut into 4-µm-thick sections. Immunostaining was conducted using the Elivision™ Plus method (EliVision™ Super KIT9922; Fuzhou Maixin Biotech. Co., Ltd.), with the procedure performed according to the kit instructions. All samples were deparaffinized with xylene and rehydrated with a graded ethanol series (100, 95, 90 and 85%). Citrate buffer (pH 6.0) was used for antigen retrieval. Methanol containing 3% H2O2 solution was used to block endogenous peroxidase activity (at 23˚C for 15 min without light), before 10% goat serum (OriGene Technologies, Inc.) was used to block nonspecific proteins (at 23˚C for 15 min). Anti-SPC24 antibody (cat. no. ab169786) primary antibody was added at a dilution of 1:250 and the sections were subsequently incubated overnight at 4˚C. After washing in PBS, all sections were incubated with biotinylated secondary antibodies (1:100, cat. no. SP-9000; OriGene Technologies, Inc.) at 23˚C for 25 min. All sections were stained with horseradish-labeled chains of ovalbumin working fluid (OriGene Technologies, Inc.) at 23˚C for 15 min, then were washed in PBS for 5 min (repeated 3 times). The images were allowed to develop in diaminobenzidine DAB substrate Kit (ZLI9018; Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd.). Finally, all sections were re-dyed with hematoxylin for 1 min at 23˚C and mounted with neutral resin. The degree of immunohistochemistry (IHC) staining was evaluated by two independent blinded pathologists (light microscope; cat. no. BX53+DP80; Olympus Corporation) observing ≥20 fields of view in each slide. The results were analyzed by an immunoreactivity score (IRS) system based on staining intensity and cell staining proportion data. The staining intensity was scored from 0-3 as follows: 0=unstained; 1=weakly stained; 2=moderately stained and 3=strongly stained. The proportion of cell staining was scored on a scale from 0-4 as follows: 0=negative; 1=1-10%; 2=11-50%; 3=51-80% and 4>80%. The intensity score was multiplied by the proportion of staining to obtain an immunoreactivity score. A total score >3 was considered to be a high level of expression. A total score of 1-3 was considered as low expression and 0 was considered no expression.
Functional and pathway enrichment analysis
To analyze the identified genes at the functional level, Gene ontology (GO) analyses, Reactome pathway analyses (reactome.org/) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses of the identified genes were conducted. The biological online database tool adopted in this study for GO and KEGG analysis was the Search Tool for the Retrieval of Interacting Genes (STRING, Version: 11.0, https://string-db.org/). Identified genes were submitted and Homo sapiens was selected in the species column. Finally, the GO terms or KEGG pathways with the cut-off criteria (P<0.05) were identified to be associated with the input genes.
Statistical analysis
Software including SPSS 21.0 (IBM Corp.), GraphPad Prism 5 (Graph Pad Software, Inc.) and MedCalc. v9.2.0.1 (MedCalc, Inc.) were used to perform statistical analyses. Unpaired t-tests were used to compare quantitative sample data from the TCGA. Data are presented in the form of the mean ± SD. Two paired-group (PCa/BPH/prostatitis tissues and adjacent/normal tissues) comparisons were conducted using χ2 test with Fisher's exact test. Association between SPC24 expression and clinicopathological factors was also determined using χ2 test. A P-value of <0.05 was considered statistically significant. Overall survival (OS) and Disease-free survival (DFS) times were stratified according to median expression level (high and low) of SPC24 (conducted in GEPIA version 2017, http://gepia.cancer-pku.cn/). Survival analyses were conducted and visualized using Kaplan-Meier analysis and log-rank test. The diagnostic ability was evaluated using receiver operating characteristic curve (ROC). The sensibility and specificity were obtained at an optimal cut-off with the max Youden index. Based on mRNA data (quantified as RSEM to estimate gene and isoform expression levels from RNA-Seq data; deweylab.github.io/RSEM/README.html) extracted from TCGA database, diagnostic ability of two combined models was also analyzed by ROC curves using binary logistic regression. Combined models acquired using binary logistic regression were: SPC24 + BUB1: Y=SPC24+BUB1*0.849/1.230; SPC24 + NDC80: Y=SPC24+NDC80*(-1.022)/1.486
Results
SPC24 analyses in TCGA dataset
The expression of SPC24 in PCa was significantly higher than that in normal tissues (P<0.0001; Fig. 1A). In addition, higher SPC24 expression was found in older PCa patients (>60), higher Gleason scores (8-10), and with higher PSA values (>0.1; Table I). Increased SPC24 was also associated with lymph node metastasis (Table I). An ROC curve was plotted to further evaluate the diagnostic value of SPC24 and the AUC value of SPC24 was 0.821 (P<0.05). Based on the maximum Youden index discriminating patients with PCa from controls, an optimal cut-off value of 2.06 was obtained, and its sensitivity and specificity for predicting PCa were calculated to be 86.54 and 67.07%, respectively (Fig. 1B). Survival analyses also suggested that high SPC24 expression was associated with negative outcomes in PCa patients (P<0.05: Fig. 1C and D).
Table IAssociation between SPC24 and clinicopathologic characteristics in The Cancer Genome Atlas PRAD database (n=498). |
Expression of SPC24 in BPH, PCa, prostatitis and adjacent/normal tissues
To further confirm the expression of SPC24 in PCa, qPCR, western blot and IHC were performed in 35 PCa tissues and paired adjacent tissues from Chinese patients with PCa. Increased SPC24 expression was found in PCa tissues compared with adjacent tissues (P<0.05: Figs. 2 and 3). qPCR analysis also suggested that SPC24 expression was higher in PCa than in BPH and adjacent tissues (P<0.05; Fig. 2A). IHC (Fig. 3B) indicated that 13 of the 35 PCa patients had high SPC24 expression, while the remaining 22 had low expression. Of the 40 BPH patients, 11 had high SPC24 expression and the remaining 29 had low or no expression. Additionally, 5 of the 12 patients with prostatitis had high levels of SPC24 expression, while the remaining had no expression. SPC24 was expressed at low levels or not expressed in both the adjacent tissues (35/35) and the normal tissues (9/9; P<0.05; Fig. 3B; Table II). These results indicated that SPC24 expression was higher in PCa tissues than in adjacent tissues, which was also confirmed by western blot (Fig. 3A). Higher SPC24 levels in BPH tissues than in the adjacent tissues were also found, while there was no difference between PCa and BPH in SPC24 expression (Fig. 3; Table II).
Table IIExpression of SPC24 in PCa tissues, adjacent tissues, BPH tissues, prostatitis tissues,and normal prostate tissues. |
Association between SPC24 expression and clinical features of patients with PCa/BPH/prostatitis
In Chinese patients with PCa, high expression of SPC24 was associated with high Gleason stage (IV and V; P<0.05). Other clinical features in PCa, including ethnicity, age, smoking, drinking, PSA, urea, creatinine, lithic acid, HCO3-, and creatinine clearance, were not correlated with SPC24 expression (Table III). In BPH tissues, SPC24 levels were associated with ethnicity and CrCl (P<0.05; Table IV). However, there was no association between SPC24 and other clinical factors in prostatitis.
Table IIIAssociation between SPC24 and clinicopathological characteristics in prostate cancer tissues (n=35). |
Table IVAssociation between SPC24 and clinicopathological characteristics in benign prostatic hypertrophic tissues (n=40). |
Gene ontology and pathway functional enrichment analysis
As shown in Table V, GO annotation and pathway enrichment analysis suggested that SPC24 and its interactors were related to several biological processes, including cell division and cell cycle. In addition, SPC24 and its interactors were related to terms suggesting they were located in the nucleus, cytosol and intracellular organelle components of the cell. Reactome pathway analysis indicated that SPC24 participated in many pathways, as it was associated with the terms resolution of sister chromatid cohesion, amplification of signal from unattached kinetochores via a MAD2 inhibitory signal, cell cycle checkpoints, RHO GTPases activate formins, separation of sister chromatids and signal transduction. The protein-protein interaction (PPI) network is shown in Fig. 4.
Table VGene ontology analyses, reactome pathway analyses and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses of SPC24. |
Increased expression of SPC24 interactors
In PCa samples, qPCR results showed that expression of SPC24 interacting proteins (BUB1 and NDC80) was higher than that in the adjacent tissues (Fig. 2B and C). Based on the mRNA data from TCGA database, two combined models via SPC24 were built to obtain better diagnosis efficiency for PCa using binary logistic regression (Fig. 5). The ROC curve of combined model of SPC24 and BUB1 was generated, and the sensitivity and specificity were 88.2 and 80.8%, respectively. The AUC was 0.894. The ROC curve of combined model of SPC24 and NDC80 had a AUC of 0.905 and the sensitivity and specificity were 84.7 and 96.2%, respectively (Table VI).
Table VISensitivity and specificity to diagnose prostate cancer with SPC24 and its interacting proteins. |
Discussion
As a subunit of the NDC80 complex, NUF2 is highly expressed in a variety of tumors and has also been shown to correlate with poor prognosis (19,20). It has been reported that SPC24 and SPC25 are required to establish and maintain kinetochore-microtubule attachments and metaphase alignment and are essential for chromosomal movement to the spindle poles during anaphase (21). High expression of SPC25 has been found in PCa (22); however, the impact of SPC24 in PCa remains to be elucidated.
The present study analyzed the TCGA database to evaluate the possible diagnostic value of SPC24 in PCa and the associations between SPC24 and clinical characteristics. The results of the TCGA dataset analysis indicated that SPC24 was highly expressed in PCa tissues compared to normal tissues. Further, results demonstrated that the high expression of SPC24 was associated with increased age, PSA value, lymph node metastasis and Gleason score, without an association with tumor level and laterality. Furthermore, analysis of a subset of clinical specimens by RT-qPCR, western blotting and immunohistochemistry demonstrated that SPC24 was significantly up-regulated in PCa tissues and BPH tissues compared to the adjacent tissues. In prostatitis tissues, SPC24 was also expressed at an increased level when compared to that in normal tissues. Future studies should include more prostate samples of patients from different nationality. The results of the present study suggested that increased SPC24 may participate in many prostatic diseases, including prostatitis, BPH and PCa.
In order to explore the potential mechanism of SPC24 and its interacting proteins in the development of prostatic diseases, bioinformatics analysis was necessary. In the present study, GO and pathway functional enrichment analysis suggested that SPC24 and its interacting proteins may participate in the cell cycle and cell division, which indicated that abnormal expression of SPC24 and its interacting proteins may interfere with the cell cycle and cell division. As loss of cell cycle control is one of the most important hallmarks of cancer (23), increased expression of SPC24 and its interacting proteins may also support the concept that there is an irregular cell cycle in PCa cells. Furthermore, multiple predicted pathways containing SPC24 determined from analysis in the study also hinted that SPC24, cooperating with its interacting proteins, could take part in many other biological processes. Validation of these predicted pathways needs to be performed in the future. Both SPC24 and SPC25 are linked with these predicted pathways. In terms of structure of protein complex, SPC24 and SPC25 fold tightly together into a single globular entity with pseudo-2-fold symmetry in vivo (24,25). In terms of protein function, SPC24 is required for meiotic kinetochore-microtubule attachment and production of euploid eggs and SPC25 is required for chromosome alignment, spindle formation and proper spindle checkpoint signaling during meiosis (26,27). These studies imply that SPC24 and SPC25 are closely related, not only in structure, but also in function. In the present study, multiple predicted pathways which all contained SPC24 and SPC25suggest a close interaction between SPC24 and SPC25. However, the effect of SPC24/SPC25 complex in prostatic diseases remains unclear. The potential role of SPC24/SPC25 complex in prostatic diseases also requires further study. Dysregulation of the kinetochore may result in chromosome aneuploidy and instability, resulting from a guidance defect for proper and accurate chromosome segregation during mitosis (28,29). NDC80 is known to be the core component of the NDC80 centromere complex and the protein interaction partner of SPC24(1). In addition, high-level expression of NDC80 was found in Human PCa tissues and increased NDC80 expression can also enhance the proliferation, migration, and invasion ability of PCa cell lines (30). It has been reported that SPC24 expression is upregulated in NDC80-overexpressing cancer cells (31). Therefore, in the present study experiments were conducted to verify NDC80 expression levels in the clinical specimens. The qPCR results showed that the level of NDC80 mRNA was higher in PCa tissues than in adjacent tissues, which was also related to expression of SPC24. The formation of proper kinetochore-microtubule attachments facilitates the procedural separation of chromosomes. The NDC80 protein is responsible for lateral attachment of microtubules, helping establish end-on attachment by indirectly interacting with the microtubule plus end through recruitment of microtubule-associated proteins (32). However, overproduction of NDC80 leads to the absorption of its interacting proteins by binding to the internal loop, blocking its interacting proteins from connecting to microtubules. This sequestration impedes mitotic progression and leads to chromosome mis-segregation, resulting in the formation of aneuploid progenies. Aneuploidy promotes tumorigenesis (33). In the present study, increased BUB1 mRNA was detected in PCa specimens. It is known that there is a surveillance system in the eukaryotic cell, the spindle assembly checkpoint (SAC), which can identify incorrectly attached or unattached kinetochores to ensure faithful chromosome segregation. The SAC consists of six components (MPH1, MAD1, MAD2, MAD3, BUB1 and BUB3). Upon SAC activation, BUB1 and other SAC components arrive at the kinetochore, generating a ‘wait-anaphase’ signal to prevent mitotic progression until all kinetochores are properly attached to microtubules emanating from the opposite spindle poles (34,35). However, when BUB1 is over-expressed, it causes mitotic errors of chromosome misalignment and lagging and resulted in near-diploid aneuploidies and tumor formation (36). These results suggest coordination among NDC80, BUB1, and SPC24 in PCa-genesis. Patients with elevated serum PSA levels are not diagnosed accurately with PCa (13). To diagnose PCa more accurately, two combined models via SPC24 mRNA were established. The AUC of two combined ROC curves was bigger than that of single SPC24, which demonstrated that combined models between SPC24 and its interacting partners could provide a discriminatory ability to diagnose PCa. At an optimal cut-off point with the maximum Youden index, the sensitivity and specificity of two combined ROC curves were different. A combined model of SPC24 and BUB1 had the highest sensitivity, while a combined model of SPC24 and NDC80 had the highest specificity. This result might be caused by different diagnostic ability of single BUB1 and NDC80, which will require further study. Compared to other previously reported PCa diagnosis markers, such as serum PSA (AUC: 0.52; sensitivity: 58%; specificity: 72%), prostate cancer antigen 3 (PCA3) mRNA (AUC: 0.865; sensitivity: 94.9%; specificity: 60.1%), and a combined model of PSA, PCA3, human glandular kallikrein 2 (HK2) (AUC: 0.667; sensitivity: 55.8%; specificity: 82.5%) (37-39), a diagnostic model of SPC24 combining BUB1/NDC80 could provide better diagnostic ability for PCa with a bigger AUC (>0.89), higher sensitivity and higher specificity (both over 80%). To the best of our knowledge, this study was the first to demonstrate that SPC24 was significantly up-regulated in BPH, prostatitis and PCa. SPC24 and its interactors may participate in some biological processes and many reactome pathways, including cohesion and separation of sister chromatids. In conclusion, SPC24 may serve a carcinogenic role in the development of PCa and may act as a new diagnostic and potential therapeutic target for PCa. The diagnostic and prognostic values of SPC24 and its possible therapeutic applications are worthy of further investigation.
Acknowledgements
Prof. Jian Qin from school of public health, Guangxi Medical University was invited to check the statistical methods in our study.
Funding
Funding: This work was supported by the National Natural Science Foundation of China (grant nos. 81560130 and 21505025) and the Guangxi Nature Science Foundation (grant nos. 2018GXNSFAA281033, 2016GXNSFDA380010 and 2013GXNSFGA019005).
Availability of data and materials
The datasets used and/or analyzed during the present study are available from the corresponding author on reasonable request.
Authors' contributions
CL and XY made substantial contributions to conception and design and agreed to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work were appropriately investigated and resolved. SC, XW and SZ wrote and revised the manuscript, performed the experiments and analyzed and interpreted scientific data. SC and SQ confirmed the authenticity of all the raw data. HL, SQ, JL and WJ performed IHC, qPCR and western blot analysis. MS, YT, HL, WS and SL participated in data collection and analysis. All authors read and approved the final manuscript.
Ethics approval and consent to participate
The present study was approved by the Ethics Committee of The First Affiliated Hospital of Guangxi Medical University (Nanning, China).
Patient consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.
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