Interleukin (IL)-4 and IL-17 synergistically stimulate IL-6 secretion in human colonic myofibroblasts

  • Authors:
    • Akira Andoh
    • Kazunori Hata
    • Yoshio Araki
    • Yoshihide Fujiyama
    • Tadao Bamba
  • View Affiliations

  • Published online on: November 1, 2002     https://doi.org/10.3892/ijmm.10.5.631
  • Pages: 631-634
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Abstract

There is increasing evidence that interleukin (IL)-4 can aid in Th1-type inflammatory responses in chronic colitis models. In this study, we evaluated the effects of IL-4 and/or IL-17 on IL-6 secretion in human colonic myofibroblasts. IL-6 secretion was determined by ELISA and Northern blotting. IL-6 secretion was rapidly induced by either IL-4 or IL-17. IL-17 induced IL-6 mRNA expression within 1 h after stimulation, and reached a maximum at 3 h. IL-6 mRNA induction by IL-4 occurred more rapidly. A maximum induction of IL-6 mRNA by IL-4 was observed at 1 h after stimulation, and this was rapidly decreased. The combination of IL-4 plus IL-17 greatly enhanced IL-6 secretion and mRNA expression. In conclusion, IL-4, in particular IL-4 plus IL-17, induced IL-6 secretion in human colonic myofibroblasts. Th2 immune responses might play an important role in the pathogenesis of gut inflammation.

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November 2002
Volume 10 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Andoh A, Hata K, Araki Y, Fujiyama Y and Bamba T: Interleukin (IL)-4 and IL-17 synergistically stimulate IL-6 secretion in human colonic myofibroblasts. Int J Mol Med 10: 631-634, 2002
APA
Andoh, A., Hata, K., Araki, Y., Fujiyama, Y., & Bamba, T. (2002). Interleukin (IL)-4 and IL-17 synergistically stimulate IL-6 secretion in human colonic myofibroblasts. International Journal of Molecular Medicine, 10, 631-634. https://doi.org/10.3892/ijmm.10.5.631
MLA
Andoh, A., Hata, K., Araki, Y., Fujiyama, Y., Bamba, T."Interleukin (IL)-4 and IL-17 synergistically stimulate IL-6 secretion in human colonic myofibroblasts". International Journal of Molecular Medicine 10.5 (2002): 631-634.
Chicago
Andoh, A., Hata, K., Araki, Y., Fujiyama, Y., Bamba, T."Interleukin (IL)-4 and IL-17 synergistically stimulate IL-6 secretion in human colonic myofibroblasts". International Journal of Molecular Medicine 10, no. 5 (2002): 631-634. https://doi.org/10.3892/ijmm.10.5.631