Light and electron microscopic analyses of immediate and late tissue damage caused by radiofrequency ablation in porcine liver

Kuromatsu,Ryoko; Tanaka,Masatoshi; Shimauchi,Yoshihiro; Harada,Riko; Ando,Eiji; Itano,Satoshi; Kumashiro,Ryukichi; Fukuda,Shuichi; Okuda,Koji; Sata,Michio

doi:10.3892/ijmm.11.2.199

February 2003 Volume 11 Issue 2

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February 2003 Volume 11 Issue 2

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Article

Light and electron microscopic analyses of immediate and late tissue damage caused by radiofrequency ablation in porcine liver

Authors:
- Ryoko Kuromatsu
- Masatoshi Tanaka
- Yoshihiro Shimauchi
- Riko Harada
- Eiji Ando
- Satoshi Itano
- Ryukichi Kumashiro
- Shuichi Fukuda
- Koji Okuda
- Michio Sata
View Affiliations / Copyright

Affiliations: Second Department of Medicine, Kurume University School of Medicine, Kurume 830-0011, Japan. ryoko-kuro@k6.dion.ne.jp
Pages: 199-204
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Published online on: February 1, 2003

https://doi.org/10.3892/ijmm.11.2.199
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Abstract

Radiofrequency ablation (RFA) is an effective procedure for localized hepatocellular carcinoma. Contrast-enhanced CT depicts the ablated area as a hypoattenuated area without hepatic blood flow; however, light microscopy does not show obvious necrosis in the ablated area. We evaluated liver tissue changes after RFA by light microscopy and electron microscopy. The normal livers of three anesthetized pigs were coagulated using RFA after laparotomy. The liver was examined immediately, and 1 week after operation by light and electron microscopy. After RFA, the liver parenchyma surrounding the needle electrode was brown in color and surrounded by a red marginal zone separate from the normal liver parenchyma. Hematoxylin-eosin staining of the central area did not show cell necrosis, and the structures of liver sinusoids, liver cell cord and the nuclei of hepatocytes were preserved. However, electron microscopic examination of tissue immediately after RFA showed destruction of mitochondria of hepatocytes and fixation of sinusoidal cells. One week later, there was a large quantity of debris in the enlarged sinusoids, in addition to irreversible destruction of hepatocyte organelles. RFA of the porcine liver causes hepatocyte damage. This damage was not evident by light microscopy but clearly identified by electron microscopy.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Light and electron microscopic analyses of immediate and late tissue damage caused by radiofrequency ablation in porcine liver

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