Mutational analysis of IκBα in hematologic malignancies

  • Authors:
    • Yoshihiro Hatta
    • Naomichi Arima
    • Tatsuya Machino
    • Takeysohi Itoh
    • Shu Hashimoto
    • Jin Takeuchi
    • Umihiko Sawada
    • Satoshi Hayakawa
    • Tatsuo Yamamoto
    • Takashi Horie
  • View Affiliations

  • Published online on: February 1, 2003     https://doi.org/10.3892/ijmm.11.2.239
  • Pages: 239-242
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The activation of the NF-κB family of transcription factors plays a crucial role in oncogenesis. The IκB family has the ability to retain the NF-κB in an inactive complex in the cytoplasm. Recently, mutations of the IκBα gene were found in Hodgkin's lymphoma, which allows NF-κB proteins to translocate into the nucleus in an active form. In this report, we describe a mutational analysis of IκBα for primary tumor cells obtained from patients with a variety of hematologic malignancies (acute myelogenous leukemia, chronic myelogenous leukemia, myelodysplastic syndrome, hairy cell leukemia, adult T-cell leukemia, and mantle cell lymphoma) as well as 15 leukemia, lymphoma, and myeloma cell lines (HL60, U937, HEL, K562, NALM1, Jurkat, JM, MOLT4, Raji, KS1, OKM2T, OKM3T, F6T, Su9T01, and C2-2). RT-PCR, followed by direct sequencing, was performed and all samples expressed IκBα. One missense mutation was identified in a primary effusion lymphoma cell line, KS1. However, NF-κB (p65) protein was absent from the nucleus of KS1 immunohistochemically, suggesting that the mutation did not alter the function of IκBα in this case. Taken together, although it is not clear whether normal IκBα protein was expressed in hematologic malignancies, mutations of IκBα could be rare events in these diseases, except for Hodgkin's lymphoma. Alterations of other members of NF-κB/ IκB family proteins might act on the development of hematologic malignancies.

Related Articles

Journal Cover

February 2003
Volume 11 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Hatta Y, Arima N, Machino T, Itoh T, Hashimoto S, Takeuchi J, Sawada U, Hayakawa S, Yamamoto T, Horie T, Horie T, et al: Mutational analysis of IκBα in hematologic malignancies. Int J Mol Med 11: 239-242, 2003.
APA
Hatta, Y., Arima, N., Machino, T., Itoh, T., Hashimoto, S., Takeuchi, J. ... Horie, T. (2003). Mutational analysis of IκBα in hematologic malignancies. International Journal of Molecular Medicine, 11, 239-242. https://doi.org/10.3892/ijmm.11.2.239
MLA
Hatta, Y., Arima, N., Machino, T., Itoh, T., Hashimoto, S., Takeuchi, J., Sawada, U., Hayakawa, S., Yamamoto, T., Horie, T."Mutational analysis of IκBα in hematologic malignancies". International Journal of Molecular Medicine 11.2 (2003): 239-242.
Chicago
Hatta, Y., Arima, N., Machino, T., Itoh, T., Hashimoto, S., Takeuchi, J., Sawada, U., Hayakawa, S., Yamamoto, T., Horie, T."Mutational analysis of IκBα in hematologic malignancies". International Journal of Molecular Medicine 11, no. 2 (2003): 239-242. https://doi.org/10.3892/ijmm.11.2.239