Effect of apoptogenic stimuli on colon carcinoma cell lines with a different c-myc expression level

  • Authors:
    • Chiara Gorrini
    • Maddalena Donzelli
    • Alicia Torriglia
    • Rosanna Supino
    • Olivier Brison
    • Rosa Bernardi
    • Claudia Negri
    • Marco Denegri
    • Marie-France Counis
    • G. Nadia Ranzani
    • A. Ivana Scovassi
  • View Affiliations

  • Published online on: June 1, 2003     https://doi.org/10.3892/ijmm.11.6.737
  • Pages: 737-742
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Abstract

We have recently demonstrated that a high c-myc endogenous amplification level confers an apoptosis-prone phenotype to serum-deprived colon carcinoma SW613-S cells. The aim of this study was to gain new insights into the features of c-myc-dependent apoptosis, by extending our analysis to different apoptogenic stimuli. The study was carried out on clones, derived from the human colon carcinoma SW613-S cell line, which harbor different levels of endogenous c-myc amplification, and on isogenic cell lines with an enforced c-myc overexpression. Our results indicate that cells with endogenous or transfected exogenous c-myc overexpression (SW613-12A1 and -2G1mycP2Tu1 cell lines, respectively), activate the apoptotic machinery in response to the treatment with etoposide, doxorubicin and vitamin D3, which induce apoptosis through the death receptor Fas. The low levels of c-myc expression present in SW613-B3 and -B3mycC5, seem to be unable to activate Fas-mediated apoptosis, thus suggesting that only a high c-myc expression can bypass the lack of Fas receptor. Apoptosis induction mediated by DNA damage and long-term culture was independent of c-myc expression. A pathway of apoptosis characterized by the activation of the enzyme L-DNase II, was observed in both 12A1 and B3 cell lines.

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June 2003
Volume 11 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Gorrini C, Donzelli M, Torriglia A, Supino R, Brison O, Bernardi R, Negri C, Denegri M, Counis M, Ranzani GN, Ranzani GN, et al: Effect of apoptogenic stimuli on colon carcinoma cell lines with a different c-myc expression level. Int J Mol Med 11: 737-742, 2003
APA
Gorrini, C., Donzelli, M., Torriglia, A., Supino, R., Brison, O., Bernardi, R. ... Scovassi, A.I. (2003). Effect of apoptogenic stimuli on colon carcinoma cell lines with a different c-myc expression level. International Journal of Molecular Medicine, 11, 737-742. https://doi.org/10.3892/ijmm.11.6.737
MLA
Gorrini, C., Donzelli, M., Torriglia, A., Supino, R., Brison, O., Bernardi, R., Negri, C., Denegri, M., Counis, M., Ranzani, G. N., Scovassi, A. I."Effect of apoptogenic stimuli on colon carcinoma cell lines with a different c-myc expression level". International Journal of Molecular Medicine 11.6 (2003): 737-742.
Chicago
Gorrini, C., Donzelli, M., Torriglia, A., Supino, R., Brison, O., Bernardi, R., Negri, C., Denegri, M., Counis, M., Ranzani, G. N., Scovassi, A. I."Effect of apoptogenic stimuli on colon carcinoma cell lines with a different c-myc expression level". International Journal of Molecular Medicine 11, no. 6 (2003): 737-742. https://doi.org/10.3892/ijmm.11.6.737