Effects of HDL3 on the expression of matrix-degrading proteases in human endothelial cells

  • Authors:
    • Giuseppe D. Norata
    • Fabio Pellegatta
    • Anders Hamsten
    • Alberico L. Catapano
    • Per Eriksson
  • View Affiliations

  • Published online on: July 1, 2003     https://doi.org/10.3892/ijmm.12.1.73
  • Pages: 73-78
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Abstract

Modified lipoproteins have been suggested to modulate the expression of matrix-degrading proteases in the vascular wall. Since oxidized high density lipoprotein (HDL) has been found in atheromatous plaques and receptors for modified HDL are present on endothelial cells, we investigated the role of native and oxidized HDL3 on the expression of 35 proteases and their inhibitors in human endothelial cells using microarray analysis. Matrix metalloproteinase (MMP)-1, -2, -10, -13 and -14, tissue inhibitor of MMP (TIMP)-1, -2 and -3, cathepsin B and D, and cystatin C were expressed under basal conditions, of which MMP-10 and cystatin C expression have not been described before in endothelial cells. Native HDL3 increased MMP-1 and MMP-14 expression and decreased MMP-13 expression, whereas oxidized HDL3 increased PAI-1 and MMP-1 expression. The expression pattern was confirmed by quantitative real-time PCR. In summary, a large repertoire of matrix-degrading proteases is expressed in endothelial cells, an expression that can be modulated by native and oxidized HDL3.

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July 2003
Volume 12 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Norata GD, Pellegatta F, Hamsten A, Catapano AL and Eriksson P: Effects of HDL3 on the expression of matrix-degrading proteases in human endothelial cells. Int J Mol Med 12: 73-78, 2003
APA
Norata, G.D., Pellegatta, F., Hamsten, A., Catapano, A.L., & Eriksson, P. (2003). Effects of HDL3 on the expression of matrix-degrading proteases in human endothelial cells. International Journal of Molecular Medicine, 12, 73-78. https://doi.org/10.3892/ijmm.12.1.73
MLA
Norata, G. D., Pellegatta, F., Hamsten, A., Catapano, A. L., Eriksson, P."Effects of HDL3 on the expression of matrix-degrading proteases in human endothelial cells". International Journal of Molecular Medicine 12.1 (2003): 73-78.
Chicago
Norata, G. D., Pellegatta, F., Hamsten, A., Catapano, A. L., Eriksson, P."Effects of HDL3 on the expression of matrix-degrading proteases in human endothelial cells". International Journal of Molecular Medicine 12, no. 1 (2003): 73-78. https://doi.org/10.3892/ijmm.12.1.73