Measuring telomerase activity in various human tumors in routine histology and cytology

Kovács,Rita Beáta; Bollmann,Magdolna; Speich,Norbert; Bollmann,Reinhard; Bodó,Miklós; Sápi,Zoltán

doi:10.3892/ijmm.13.2.303

February 2004 Volume 13 Issue 2

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February 2004 Volume 13 Issue 2

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Article

Measuring telomerase activity in various human tumors in routine histology and cytology

Authors:
- Rita Beáta Kovács
- Magdolna Bollmann
- Norbert Speich
- Reinhard Bollmann
- Miklós Bodó
- Zoltán Sápi
View Affiliations / Copyright

Affiliations: First Department of Pathology and Experimental Cancer Research, Teaching Unit, Faculty of Medicine, Semmelweis University, Budapest, Hungary. ritabea@freemail.hu
Pages: 303-309
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Published online on: February 1, 2004

https://doi.org/10.3892/ijmm.13.2.303
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Abstract

The aim of this study was to investigate telomerase reactivation, to quantitatively measure the human telomerase reverse transcriptase (hTERT) content and telomerase activity level (TA) in routine histological and cytological samples, and to examine the relationship between these values and morphological factors. We analyzed 86 (35 cytological and 51 histological) lesions which were divided into four main groups: renal tumors, soft tissue tumors, bladder-urine and thyroid gland lesions. The relative expression of mRNA of hTERT was examined by real-time polymerase chain reaction (RT-PCR). Almost all of the renal cell carcinomas showed TA. In soft tissue tumors no correlation was seen between TA and histogenesis, aggressiveness and prognosis. Concerning cytological material a very good correlation was seen between TA and the benign or malignant nature of these tumors (92.3% specificity and 60% sensitivity). Our results indicated that TA can be used beside histology also in cytologic samples, for example in the preoperative differential diagnosis of the thyroid gland lesions and urine samples. Telomerase reactivation may not play an important role in tumorigenesis in STT. Useful observations can be made by concentrating not only on one group of diseases or localization but the unselected analysis of routine diagnostic cases can also be of help both in diagnosis and therapy.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Measuring telomerase activity in various human tumors in routine histology and cytology

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Abstract

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