Hypermethylation of the MYOD1 gene is a novel prognostic factor in patients with colorectal cancer

  • Authors:
    • Chikashi Hiranuma
    • Kazuyuki Kawakami
    • Kaeko Oyama
    • Naohiro Ota
    • Kenji Omura
    • Go Watanabe
  • View Affiliations

  • Published online on: March 1, 2004     https://doi.org/10.3892/ijmm.13.3.413
  • Pages: 413-417
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Abstract

MYOD1 promoter methylation occurs in various malignancies including colorectal cancer. We analyzed MYOD1 methylation in 80 colorectal cancer and 74 adjacent normal tissues using MethyLight, which enabled quantitative DNA methylation analysis. The measured methylation value was expressed as a percentage of methylated reference (PMR). The results were compared with clinicopathological features and patient prognosis in order to investigate whether MYOD1 methylation may serve as an independent prognostic factor of colorectal cancer. MYOD1 promoter methylation was detectable in all samples and was significantly higher in tumor compared to normal mucosa, where the median level of methylation was 5.3 PMR (range 0.03-133.4) in normal mucosa and 42.3 PMR (range 0.44-742.9) in tumor. The methylation value of MYOD1 was higher with elder patients both in normal colonic mucosa (p=0.034) and in cancer tissues (p=0.0012). Patients without MYOD1 hypermethylation showed significantly longer survival than those with hypermethylation (p=0.0077). In multivariate analysis of prognostic factors, MYOD1 hypermethylation was a significant prognostic factor (p=0.015) independent to patients' age. These results suggest that MYOD1 hypermethylation plays an important role in colorectal cancer and may be a novel prognostic factor. In addition, quantitative methylation analysis by MethyLight is encouraged for other genes showing age-related and non-cancer-specific methylation.

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March 2004
Volume 13 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Hiranuma C, Kawakami K, Oyama K, Ota N, Omura K and Watanabe G: Hypermethylation of the MYOD1 gene is a novel prognostic factor in patients with colorectal cancer. Int J Mol Med 13: 413-417, 2004
APA
Hiranuma, C., Kawakami, K., Oyama, K., Ota, N., Omura, K., & Watanabe, G. (2004). Hypermethylation of the MYOD1 gene is a novel prognostic factor in patients with colorectal cancer. International Journal of Molecular Medicine, 13, 413-417. https://doi.org/10.3892/ijmm.13.3.413
MLA
Hiranuma, C., Kawakami, K., Oyama, K., Ota, N., Omura, K., Watanabe, G."Hypermethylation of the MYOD1 gene is a novel prognostic factor in patients with colorectal cancer". International Journal of Molecular Medicine 13.3 (2004): 413-417.
Chicago
Hiranuma, C., Kawakami, K., Oyama, K., Ota, N., Omura, K., Watanabe, G."Hypermethylation of the MYOD1 gene is a novel prognostic factor in patients with colorectal cancer". International Journal of Molecular Medicine 13, no. 3 (2004): 413-417. https://doi.org/10.3892/ijmm.13.3.413