Lipidic inhibitors of human N-myristoyltransferase

  • Authors:
    • Mohammed Khysar Pasha
    • Ponniah Selvakumar
    • Lakshmikuttyamma Ashakumary
    • Mabood Qureshi
    • Frank S. Guziec
    • Jonathan R. Dimmock
    • Ronald L. Felsted
    • Constance J. Glover
    • Rajendra K. Sharma
  • View Affiliations

  • Published online on: April 1, 2004     https://doi.org/10.3892/ijmm.13.4.557
  • Pages: 557-563
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Abstract

This study was undertaken in order to identify compounds which inhibit the activity of human myristoyl-CoA:protein N-myristoyltransferase (hNMT). In particular, the structural features of such molecules which contribute to enzyme inhibition were investigated. Two groups of compounds, namely myristic acid and analogs 1-13 and derivatives of myristoyl-CoA 14-19 were evaluated. All compounds were examined using cAMP-dependent protein kinase derived peptide substrate. The IC50 values were <1 µM, between 1 and 100 µM or >100 µM in eight, four and seven compounds, respectively. Of the six myristoyl-CoA analogs, five had IC50 values in the 0.06-0.59 µM range. These molecules were examined using three additional substrates viz pp60src, MARCKS and M2 gene segment of reovirus type 3 which led to results similar to those obtained with the cAMP-dependent protein kinase substrate. On the other hand, evaluation of myristic acid and four related compounds revealed some differences in hNMT-inhibiting properties among the substrates. From the results obtained, the possible manner whereby potent inhibitors interact with the enzyme was formulated thus enabling the design of further analogs as candidate inhibitors of hNMT.

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April 2004
Volume 13 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Pasha MK, Selvakumar P, Ashakumary L, Qureshi M, Guziec FS, Dimmock JR, Felsted RL, Glover CJ and Sharma RK: Lipidic inhibitors of human N-myristoyltransferase. Int J Mol Med 13: 557-563, 2004
APA
Pasha, M.K., Selvakumar, P., Ashakumary, L., Qureshi, M., Guziec, F.S., Dimmock, J.R. ... Sharma, R.K. (2004). Lipidic inhibitors of human N-myristoyltransferase. International Journal of Molecular Medicine, 13, 557-563. https://doi.org/10.3892/ijmm.13.4.557
MLA
Pasha, M. K., Selvakumar, P., Ashakumary, L., Qureshi, M., Guziec, F. S., Dimmock, J. R., Felsted, R. L., Glover, C. J., Sharma, R. K."Lipidic inhibitors of human N-myristoyltransferase". International Journal of Molecular Medicine 13.4 (2004): 557-563.
Chicago
Pasha, M. K., Selvakumar, P., Ashakumary, L., Qureshi, M., Guziec, F. S., Dimmock, J. R., Felsted, R. L., Glover, C. J., Sharma, R. K."Lipidic inhibitors of human N-myristoyltransferase". International Journal of Molecular Medicine 13, no. 4 (2004): 557-563. https://doi.org/10.3892/ijmm.13.4.557