Cloning of the Cav 1.3 (α1D) L-type Ca2+ channel from mouse and its expression in the liver

  • Authors:
    • Toshihiro Okamoto
    • Toshiyuki Kobayashi
    • Okio Hino
  • View Affiliations

  • Published online on: April 1, 2004     https://doi.org/10.3892/ijmm.13.4.573
  • Pages: 573-576
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Abstract

Dihydropyridine (DHP) type Ca2+ channel blocker (CCB) is effective in treatment of hepatitis in man. L-type Ca2+ channel is a target of DHP-CCB, and basic studies suggest that L-type Ca2+ channel α1D-subunit (Cav 1.3) seems to be a target of drug development for the treatment of hepatitis. Mouse hepatitis model is useful to study the effect of DHP-CCB on hepatitis. In order to use mouse hepatitis model to screen DHP-CCB specific for Cav 1.3, Cav 1.3 expressed in the mouse liver should have enough structural homology with that of human Cav 1.3. cDNA of the Cav 1.3 was cloned from mouse brain by reverse transcription polymerase chain reaction. The primary structure of the mouse Cav 1.3 comprises an open reading frame of 6540 bp encoding 2180 amino acids. Liver transcript lacked 60 and 45 bases from 1497 to 1556, and from 3949 to 3993 of the sequence, respectively, due to results of an alternative splicing. The present results indicated that mouse Cav 1.3 exhibited 96% homology with human Cav 1.3 and was expressed in the liver. Thus, mouse hepatitis model seemed to be useful to screen DHP-CCB specific for Cav 1.3.

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April 2004
Volume 13 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Okamoto T, Kobayashi T and Hino O: Cloning of the Cav 1.3 (α1D) L-type Ca2+ channel from mouse and its expression in the liver. Int J Mol Med 13: 573-576, 2004
APA
Okamoto, T., Kobayashi, T., & Hino, O. (2004). Cloning of the Cav 1.3 (α1D) L-type Ca2+ channel from mouse and its expression in the liver. International Journal of Molecular Medicine, 13, 573-576. https://doi.org/10.3892/ijmm.13.4.573
MLA
Okamoto, T., Kobayashi, T., Hino, O."Cloning of the Cav 1.3 (α1D) L-type Ca2+ channel from mouse and its expression in the liver". International Journal of Molecular Medicine 13.4 (2004): 573-576.
Chicago
Okamoto, T., Kobayashi, T., Hino, O."Cloning of the Cav 1.3 (α1D) L-type Ca2+ channel from mouse and its expression in the liver". International Journal of Molecular Medicine 13, no. 4 (2004): 573-576. https://doi.org/10.3892/ijmm.13.4.573