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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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February 2005 Volume 15 Issue 2

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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February 2005 Volume 15 Issue 2

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Article

A novel component of the ubiquitin pathway, ubiquitin carboxyl extension protein 1 is overexpressed in prostate cancer

  • Authors:
    • Yon Ko
    • Tanja Hahn
    • Hong Lu
    • Zhan Lu Ma
    • Jian Chen
    • Marcus Rothe
    • Alexandra Florin
    • Stefan Fronhoffs
    • Peter Albers
    • Agapios Sachinidis
    • Hans Vetter
    • Annette Kaminski
    • Peter Behrens
    • Axel Wellmann
    • Nicolas Wernert
  • View Affiliations / Copyright

    Affiliations: Medizinische Poliklinik, University of Bonn, D-53111 Bonn, Germany. yonko@uni-bonn.de
  • Pages: 183-196
    |
    Published online on: February 1, 2005
       https://doi.org/10.3892/ijmm.15.2.183
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Abstract

Prostate cancer is among the most common tumors in industrialized nations. However, little is known about the molecular events underlying its development. In the present study we used suppressive subtraction hybridization (SSH) in combination with laser-assisted microdissection in order to compare gene expression between prostate carcinoma and the normal prostate proper. Both are mixed tissues which consist of an epithelial and a stromal compartment. We first compared mRNA (cDNA) expression by SSH and then used real-time quantitative RT-PCR analysis of microdissected tissue probes in order to verify differential expression of subtracted cDNA clones. We also used differentially expressed cDNAs for the synthesis of radiolabelled riboprobes in order to attribute differential expression to specific cell types in tissue sections by in situ hybridization. Using this approach we found an up-regulation of ubiquitin carboxyl extension protein 1 (UBCEP-1) mRNA in prostate carcinoma cells compared to the normal glandular epithelium of the prostate proper. UBCEP-1 mediated ubiquitin chain elongation may promote prostate carcinoma development by increasing via the proteasome pathway the degradation of proteins which are involved in growth inhibition or apoptosis.

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Copy and paste a formatted citation
Spandidos Publications style
Ko Y, Hahn T, Lu H, Ma Z, Chen J, Rothe M, Florin A, Fronhoffs S, Albers P, Sachinidis A, Sachinidis A, et al: A novel component of the ubiquitin pathway, ubiquitin carboxyl extension protein 1 is overexpressed in prostate cancer. Int J Mol Med 15: 183-196, 2005.
APA
Ko, Y., Hahn, T., Lu, H., Ma, Z., Chen, J., Rothe, M. ... Wernert, N. (2005). A novel component of the ubiquitin pathway, ubiquitin carboxyl extension protein 1 is overexpressed in prostate cancer. International Journal of Molecular Medicine, 15, 183-196. https://doi.org/10.3892/ijmm.15.2.183
MLA
Ko, Y., Hahn, T., Lu, H., Ma, Z., Chen, J., Rothe, M., Florin, A., Fronhoffs, S., Albers, P., Sachinidis, A., Vetter, H., Kaminski, A., Behrens, P., Wellmann, A., Wernert, N."A novel component of the ubiquitin pathway, ubiquitin carboxyl extension protein 1 is overexpressed in prostate cancer". International Journal of Molecular Medicine 15.2 (2005): 183-196.
Chicago
Ko, Y., Hahn, T., Lu, H., Ma, Z., Chen, J., Rothe, M., Florin, A., Fronhoffs, S., Albers, P., Sachinidis, A., Vetter, H., Kaminski, A., Behrens, P., Wellmann, A., Wernert, N."A novel component of the ubiquitin pathway, ubiquitin carboxyl extension protein 1 is overexpressed in prostate cancer". International Journal of Molecular Medicine 15, no. 2 (2005): 183-196. https://doi.org/10.3892/ijmm.15.2.183
Copy and paste a formatted citation
x
Spandidos Publications style
Ko Y, Hahn T, Lu H, Ma Z, Chen J, Rothe M, Florin A, Fronhoffs S, Albers P, Sachinidis A, Sachinidis A, et al: A novel component of the ubiquitin pathway, ubiquitin carboxyl extension protein 1 is overexpressed in prostate cancer. Int J Mol Med 15: 183-196, 2005.
APA
Ko, Y., Hahn, T., Lu, H., Ma, Z., Chen, J., Rothe, M. ... Wernert, N. (2005). A novel component of the ubiquitin pathway, ubiquitin carboxyl extension protein 1 is overexpressed in prostate cancer. International Journal of Molecular Medicine, 15, 183-196. https://doi.org/10.3892/ijmm.15.2.183
MLA
Ko, Y., Hahn, T., Lu, H., Ma, Z., Chen, J., Rothe, M., Florin, A., Fronhoffs, S., Albers, P., Sachinidis, A., Vetter, H., Kaminski, A., Behrens, P., Wellmann, A., Wernert, N."A novel component of the ubiquitin pathway, ubiquitin carboxyl extension protein 1 is overexpressed in prostate cancer". International Journal of Molecular Medicine 15.2 (2005): 183-196.
Chicago
Ko, Y., Hahn, T., Lu, H., Ma, Z., Chen, J., Rothe, M., Florin, A., Fronhoffs, S., Albers, P., Sachinidis, A., Vetter, H., Kaminski, A., Behrens, P., Wellmann, A., Wernert, N."A novel component of the ubiquitin pathway, ubiquitin carboxyl extension protein 1 is overexpressed in prostate cancer". International Journal of Molecular Medicine 15, no. 2 (2005): 183-196. https://doi.org/10.3892/ijmm.15.2.183
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