Effects of β-amyloid on rat neuromicrovascular endothelial cells cultured in vitro

  • Authors:
    • Marcella Folin
    • Silvia Baiguera
    • Mara Tommasini
    • Diego Guidolin
    • Maria Teresa Conconi
    • Eugenio De Carlo
    • Gastone G. Nussdorfer
    • Pier Paolo Parnigotto
  • View Affiliations

  • Published online on: June 1, 2005     https://doi.org/10.3892/ijmm.15.6.929
  • Pages: 929-935
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Several studies have shown that β-amyloid (βA) deposits are associated with damage of cerebral vessels and that in Alzheimer's disease (AD) βA peptides are cytotoxic for cerebral endothelial cells (ECs). However, little is known about the mechanisms underlying these effects of βA peptides. Hence, we have investigated the effects of βA(1-40) and βA(1-42) on rat neuromicrovascular ECs (NECs) cultured in vitro. NECs were isolated, plated (1.5x104 cells/cm2) on collagen/fibronectin-coated Petri dishes and cultured in EC growth medium MV2. After 24 h of culture, NECs were incubated with βA(1-40) and βA(1-42) (10−9 or 10−7 M) and cultured for another 3, 24 or 48 h. Cell viability was assayed by either trypan blue exclusion or by measuring redox activity (MTS assay). Cell proliferation was assessed by measuring the incorporation of 5'-bromo-2'-deoxyuridine into DNA, cell apoptosis by TUNEL assay and cell necrosis by evaluating the release of lactate dehydrogenase. The morphology of cultured NECs was examined by transmission electron microscopy. Other NECs were plated (2.5x104 cells/cm2) on Matrigel coated-wells and incubated for 18 h in the presence or absence of βA peptides for in vitro angiogenesis assay. βA peptides significantly decreased NEC viability and enhanced cell apoptosis and necrosis rates. NEC proliferation was not significantly affected. The effects were seen after an incubation period of 3 h (and also 24 h in the case of the redox activity) but not 48 h and βA(1-42) was much more effective in its toxic effects than βA(1-40). NECs incubated for 24 h with βA peptides displayed ultrastructural signs of cell degeneration. βA peptides prevented NECs cultured on Matrigel to form a capillary-like network and image analysis showed that the downloading of dimensional and topological parameters of the meshwork was significant only in the case of the incubation with βA(1-42). Collectively our findings allow us to conclude that i) βA peptides exert a marked toxic effect on cultured NECs, which conceivably reduces their in vitro angiogenic activity; ii) βA(1-42) is the more toxic form, which could suggest its main role in the pathogenesis of cerebral vessel lesions in AD and iii) the maximum toxic action occurs after a short incubation period, which could be explained by assuming that βA peptides are unable to accumulate in NECs due to their rapid degradation, thereby allowing NECs to fully recover within 48 h.

Related Articles

Journal Cover

June 2005
Volume 15 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Folin M, Baiguera S, Tommasini M, Guidolin D, Conconi MT, De Carlo E, Nussdorfer GG and Parnigotto PP: Effects of β-amyloid on rat neuromicrovascular endothelial cells cultured in vitro. Int J Mol Med 15: 929-935, 2005
APA
Folin, M., Baiguera, S., Tommasini, M., Guidolin, D., Conconi, M.T., De Carlo, E. ... Parnigotto, P.P. (2005). Effects of β-amyloid on rat neuromicrovascular endothelial cells cultured in vitro. International Journal of Molecular Medicine, 15, 929-935. https://doi.org/10.3892/ijmm.15.6.929
MLA
Folin, M., Baiguera, S., Tommasini, M., Guidolin, D., Conconi, M. T., De Carlo, E., Nussdorfer, G. G., Parnigotto, P. P."Effects of β-amyloid on rat neuromicrovascular endothelial cells cultured in vitro". International Journal of Molecular Medicine 15.6 (2005): 929-935.
Chicago
Folin, M., Baiguera, S., Tommasini, M., Guidolin, D., Conconi, M. T., De Carlo, E., Nussdorfer, G. G., Parnigotto, P. P."Effects of β-amyloid on rat neuromicrovascular endothelial cells cultured in vitro". International Journal of Molecular Medicine 15, no. 6 (2005): 929-935. https://doi.org/10.3892/ijmm.15.6.929