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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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June 2005 Volume 15 Issue 6

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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Article

Cloning and characterization of a novel intronless lactate dehydrogenase gene in human testis

  • Authors:
    • Hui Wang
    • Zuomin Zhou
    • Li Lu
    • Zhiyang Xu
    • Jiahao Sha

  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, P.R. China
  • Pages: 949-953
    |
    Published online on: June 1, 2005
       https://doi.org/10.3892/ijmm.15.6.949
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Abstract

Using cDNA microarray hybridization from a human testicular cDNA library, one gene named lactate dehydrogenase A-like gene (LDHL, also known as LDHL6B) was cloned. LDHL exhibited 3.8-fold difference at expression level between adult and fetal human testes. The full cDNA length of LDHL is 1680 bp and had a 1145 bp open reading frame, which encoded a 41.9 kDa protein of 381 amino acids. Sequence analysis showed that LDHL harbors all the domains (one lactate/malate dehydrogenase, NAD binding domain and one lactate/malate dehydrogenase, α/β C-terminal domain) in lactate dehydrogenase gene family. Blasting human genome database localized LDHL to human chromosome 15q22.2 and it was an intronless gene. Results of multiple-tissue PCR and real-time PCR showed that LDHL expressed mainly in testis and its mRNA abundance was testis development- related. In summary, LDHL is believed to be involved in testis development and spermatogenesis.

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Copy and paste a formatted citation
Spandidos Publications style
Wang H, Zhou Z, Lu L, Xu Z and Sha J: Cloning and characterization of a novel intronless lactate dehydrogenase gene in human testis. Int J Mol Med 15: 949-953, 2005.
APA
Wang, H., Zhou, Z., Lu, L., Xu, Z., & Sha, J. (2005). Cloning and characterization of a novel intronless lactate dehydrogenase gene in human testis. International Journal of Molecular Medicine, 15, 949-953. https://doi.org/10.3892/ijmm.15.6.949
MLA
Wang, H., Zhou, Z., Lu, L., Xu, Z., Sha, J."Cloning and characterization of a novel intronless lactate dehydrogenase gene in human testis". International Journal of Molecular Medicine 15.6 (2005): 949-953.
Chicago
Wang, H., Zhou, Z., Lu, L., Xu, Z., Sha, J."Cloning and characterization of a novel intronless lactate dehydrogenase gene in human testis". International Journal of Molecular Medicine 15, no. 6 (2005): 949-953. https://doi.org/10.3892/ijmm.15.6.949
Copy and paste a formatted citation
x
Spandidos Publications style
Wang H, Zhou Z, Lu L, Xu Z and Sha J: Cloning and characterization of a novel intronless lactate dehydrogenase gene in human testis. Int J Mol Med 15: 949-953, 2005.
APA
Wang, H., Zhou, Z., Lu, L., Xu, Z., & Sha, J. (2005). Cloning and characterization of a novel intronless lactate dehydrogenase gene in human testis. International Journal of Molecular Medicine, 15, 949-953. https://doi.org/10.3892/ijmm.15.6.949
MLA
Wang, H., Zhou, Z., Lu, L., Xu, Z., Sha, J."Cloning and characterization of a novel intronless lactate dehydrogenase gene in human testis". International Journal of Molecular Medicine 15.6 (2005): 949-953.
Chicago
Wang, H., Zhou, Z., Lu, L., Xu, Z., Sha, J."Cloning and characterization of a novel intronless lactate dehydrogenase gene in human testis". International Journal of Molecular Medicine 15, no. 6 (2005): 949-953. https://doi.org/10.3892/ijmm.15.6.949
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