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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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September 2005 Volume 16 Issue 3

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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September 2005 Volume 16 Issue 3

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Article

Co-expression of the complement regulatory proteins human DAF and CD59 with an IRES-mediated dicistronic mammalian vector enhances their cell protective effects

  • Authors:
    • Li Xu
    • Huanjie Shao
    • Wenlan Wu
    • Zhijian Cao
    • Zhouzhou Zhao
    • Hui Liu
    • Dahe Jiang
    • Xin Mao
    • Wenxin Li

  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Virology, College of Life Science, Wuhan University, Wuhan 430072, P.R. China
  • Pages: 409-414
    |
    Published online on: September 1, 2005
       https://doi.org/10.3892/ijmm.16.3.409
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Abstract

The human complement regulatory proteins (hCRPs) decay accelerating factor (DAF/CD55) and protectin CD59 transfected into non-human cells could confer protection against human complement. The combination of DAF and CD59 would be an effective strategy to help overcome host complement-induced hyperacute rejection in xenotransplantation. We constructed a dicistronic mammalian expression vector pcDNA3-CD59IRESDAF by using the internal ribosomal entry sites (IRES) of the encephalomyocarditis virus (EMCV). RT-PCR, Western blotting and immunofluorescence microscopic analysis demonstrated that the EMCV IRES allowed for efficient co-expression of hCD59 and hDAF on the surface of NIH/3T3 cells transfected stably with pcDNA3-CD59IRESDAF. Human complement-mediated cytolysis assays showed that co-expressed DAF and CD59 proteins could provide more significant protection against complement-mediated cytolysis than either hCD59 or hDAF alone. These results suggest that IRES containing polycistronic vector should improve the efficiency and effectiveness of multi-gene delivery and that the construct pcDNA3-CD59IRESDAF vector has potential therapeutic value for effectively controlling complement activation and for preventing hyperacute rejection in clinical gene therapy.

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Copy and paste a formatted citation
Spandidos Publications style
Xu L, Shao H, Wu W, Cao Z, Zhao Z, Liu H, Jiang D, Mao X and Li W: Co-expression of the complement regulatory proteins human DAF and CD59 with an IRES-mediated dicistronic mammalian vector enhances their cell protective effects. Int J Mol Med 16: 409-414, 2005.
APA
Xu, L., Shao, H., Wu, W., Cao, Z., Zhao, Z., Liu, H. ... Li, W. (2005). Co-expression of the complement regulatory proteins human DAF and CD59 with an IRES-mediated dicistronic mammalian vector enhances their cell protective effects. International Journal of Molecular Medicine, 16, 409-414. https://doi.org/10.3892/ijmm.16.3.409
MLA
Xu, L., Shao, H., Wu, W., Cao, Z., Zhao, Z., Liu, H., Jiang, D., Mao, X., Li, W."Co-expression of the complement regulatory proteins human DAF and CD59 with an IRES-mediated dicistronic mammalian vector enhances their cell protective effects". International Journal of Molecular Medicine 16.3 (2005): 409-414.
Chicago
Xu, L., Shao, H., Wu, W., Cao, Z., Zhao, Z., Liu, H., Jiang, D., Mao, X., Li, W."Co-expression of the complement regulatory proteins human DAF and CD59 with an IRES-mediated dicistronic mammalian vector enhances their cell protective effects". International Journal of Molecular Medicine 16, no. 3 (2005): 409-414. https://doi.org/10.3892/ijmm.16.3.409
Copy and paste a formatted citation
x
Spandidos Publications style
Xu L, Shao H, Wu W, Cao Z, Zhao Z, Liu H, Jiang D, Mao X and Li W: Co-expression of the complement regulatory proteins human DAF and CD59 with an IRES-mediated dicistronic mammalian vector enhances their cell protective effects. Int J Mol Med 16: 409-414, 2005.
APA
Xu, L., Shao, H., Wu, W., Cao, Z., Zhao, Z., Liu, H. ... Li, W. (2005). Co-expression of the complement regulatory proteins human DAF and CD59 with an IRES-mediated dicistronic mammalian vector enhances their cell protective effects. International Journal of Molecular Medicine, 16, 409-414. https://doi.org/10.3892/ijmm.16.3.409
MLA
Xu, L., Shao, H., Wu, W., Cao, Z., Zhao, Z., Liu, H., Jiang, D., Mao, X., Li, W."Co-expression of the complement regulatory proteins human DAF and CD59 with an IRES-mediated dicistronic mammalian vector enhances their cell protective effects". International Journal of Molecular Medicine 16.3 (2005): 409-414.
Chicago
Xu, L., Shao, H., Wu, W., Cao, Z., Zhao, Z., Liu, H., Jiang, D., Mao, X., Li, W."Co-expression of the complement regulatory proteins human DAF and CD59 with an IRES-mediated dicistronic mammalian vector enhances their cell protective effects". International Journal of Molecular Medicine 16, no. 3 (2005): 409-414. https://doi.org/10.3892/ijmm.16.3.409
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