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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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September 2005 Volume 16 Issue 3

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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September 2005 Volume 16 Issue 3

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Article

Adrenomedullin and vascular endothelium growth factor genes are overexpressed in the regenerating rat adrenal cortex, and AM and VEGF reciprocally enhance their mRNA expression in cultured rat adrenocortical cells

  • Authors:
    • Giovanna Albertin
    • Marcin Rucinski
    • Gianni Carraro
    • Myriam Forneris
    • Paola Andreis
    • Ludwik K. Malendowicz
    • Gastone G. Nussdorfer
  • View Affiliations / Copyright

    Affiliations: Department of Human Anatomy and Physiology, Section of Anatomy, Via Gabelli 65, I-35121 Padova, Italy
  • Pages: 431-435
    |
    Published online on: September 1, 2005
       https://doi.org/10.3892/ijmm.16.3.431
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Abstract

Adrenomedullin (AM) is an endogenous regulatory peptide, which exerts growth promoting and neoangiogenic actions in several normal and neoplastic tissues. Evidence has been provided that AM and the pro-angiogenic peptide vascular endothelium growth factor (VEGF) are expressed in the adrenal gland, and we investigated whether their gene transcription is modified in a model of rapid rat adrenal growth, namely regeneration after enucleation and contralateral adrenalectomy. Real-time polymerase chain reaction (PCR) showed that AM and VEGF mRNAs were significantly increased in regenerating adrenals with respect to the intact adrenocortical tissue of sham-operated control rats. Subsequent studies were carried out on dispersed rat adrenocortical cells cultured in vitro for 72 h. Conventional PCR demonstrated that cultured cells expressed AM and VEGF mRNAs. Moreover, real-time PCR showed that 24 h exposure to 10-8 M AM or 50 ng/ml VEGF significantly raised the expression of VEGF and AM, respectively, without affecting that of their own mRNA, suggesting the occurence of autocrine-paracrine up-regulatory mechanisms. Taken together, our findings allow us to conceive that the coordinate up-regulation of AM and VEGF expression may favor cell proliferation and neoangiogenesis, thereby leading to a rapid restoration of morphology and function of regenerating adrenals.

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Copy and paste a formatted citation
Spandidos Publications style
Albertin G, Rucinski M, Carraro G, Forneris M, Andreis P, Malendowicz LK and Nussdorfer GG: Adrenomedullin and vascular endothelium growth factor genes are overexpressed in the regenerating rat adrenal cortex, and AM and VEGF reciprocally enhance their mRNA expression in cultured rat adrenocortical cells. Int J Mol Med 16: 431-435, 2005.
APA
Albertin, G., Rucinski, M., Carraro, G., Forneris, M., Andreis, P., Malendowicz, L.K., & Nussdorfer, G.G. (2005). Adrenomedullin and vascular endothelium growth factor genes are overexpressed in the regenerating rat adrenal cortex, and AM and VEGF reciprocally enhance their mRNA expression in cultured rat adrenocortical cells. International Journal of Molecular Medicine, 16, 431-435. https://doi.org/10.3892/ijmm.16.3.431
MLA
Albertin, G., Rucinski, M., Carraro, G., Forneris, M., Andreis, P., Malendowicz, L. K., Nussdorfer, G. G."Adrenomedullin and vascular endothelium growth factor genes are overexpressed in the regenerating rat adrenal cortex, and AM and VEGF reciprocally enhance their mRNA expression in cultured rat adrenocortical cells". International Journal of Molecular Medicine 16.3 (2005): 431-435.
Chicago
Albertin, G., Rucinski, M., Carraro, G., Forneris, M., Andreis, P., Malendowicz, L. K., Nussdorfer, G. G."Adrenomedullin and vascular endothelium growth factor genes are overexpressed in the regenerating rat adrenal cortex, and AM and VEGF reciprocally enhance their mRNA expression in cultured rat adrenocortical cells". International Journal of Molecular Medicine 16, no. 3 (2005): 431-435. https://doi.org/10.3892/ijmm.16.3.431
Copy and paste a formatted citation
x
Spandidos Publications style
Albertin G, Rucinski M, Carraro G, Forneris M, Andreis P, Malendowicz LK and Nussdorfer GG: Adrenomedullin and vascular endothelium growth factor genes are overexpressed in the regenerating rat adrenal cortex, and AM and VEGF reciprocally enhance their mRNA expression in cultured rat adrenocortical cells. Int J Mol Med 16: 431-435, 2005.
APA
Albertin, G., Rucinski, M., Carraro, G., Forneris, M., Andreis, P., Malendowicz, L.K., & Nussdorfer, G.G. (2005). Adrenomedullin and vascular endothelium growth factor genes are overexpressed in the regenerating rat adrenal cortex, and AM and VEGF reciprocally enhance their mRNA expression in cultured rat adrenocortical cells. International Journal of Molecular Medicine, 16, 431-435. https://doi.org/10.3892/ijmm.16.3.431
MLA
Albertin, G., Rucinski, M., Carraro, G., Forneris, M., Andreis, P., Malendowicz, L. K., Nussdorfer, G. G."Adrenomedullin and vascular endothelium growth factor genes are overexpressed in the regenerating rat adrenal cortex, and AM and VEGF reciprocally enhance their mRNA expression in cultured rat adrenocortical cells". International Journal of Molecular Medicine 16.3 (2005): 431-435.
Chicago
Albertin, G., Rucinski, M., Carraro, G., Forneris, M., Andreis, P., Malendowicz, L. K., Nussdorfer, G. G."Adrenomedullin and vascular endothelium growth factor genes are overexpressed in the regenerating rat adrenal cortex, and AM and VEGF reciprocally enhance their mRNA expression in cultured rat adrenocortical cells". International Journal of Molecular Medicine 16, no. 3 (2005): 431-435. https://doi.org/10.3892/ijmm.16.3.431
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