Transient expression of keratin 19 is induced in originally negative interfollicular epidermal cells by adhesion of suspended cells

  • Authors:
    • Barbora DvoRánková
    • Karel Smetana
    • Martin Chovanec
    • LukáS Lacina
    • Jirí Stork
    • Zuzana Plzáková
    • Mariana GaloviCová
    • Hans-Joachim Gabius
  • View Affiliations

  • Published online on: October 1, 2005     https://doi.org/10.3892/ijmm.16.4.525
  • Pages: 525-531
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Keratin 19 and nuclear reactivity to an endogenous lectin, galectin-1, represent a potential marker of epidermal stem cells. We detected expression of keratin 19 and nuclear binding sites for galectin-1 in adult cells migrating from the hair follicle, where cells expressing keratin 19 are located in the bulge region. The results were compared with the expression of both markers in cells adhering from suspension prepared from the interfollicular epidermis without keratin-19-positive cells and with nuclear binding sites for galectin-1. The results were compared with data from basal cell carcinomas. All cells were analyzed concerning size, as it is known that cell diameter influences the clonogenic potential of keratinocytes. The major result of this study is the observation of transient expression of keratin 19 and nuclear galectin-1 binding sites in originally negative interfollicular epidermal cells induced by adhesion. These cells were very small in size, similar to basal cells of the interfollicular epidermis or the bulge region of the hair follicle. The influence of the suspension regimen on β1-integrin expression, cell diameter and growth was also monitored. A population of cells highly positive for β1 integrin of the same diameter as keratin-19-positive cells insensitive to induction of terminal differentiation by lack of anchorage was characterized. Cells of the same size were also observed in the keratin-19-positive cells of basal cell carcinomas. In conclusion, the expression of poor levels of differentiation induced by cell adhesion is transient. Also, keratin 19 expression should not be exclusively regarded as a marker of stem cell activity.

Related Articles

Journal Cover

October 2005
Volume 16 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
DvoRánková B, Smetana K, Chovanec M, Lacina L, Stork J, Plzáková Z, GaloviCová M and Gabius H: Transient expression of keratin 19 is induced in originally negative interfollicular epidermal cells by adhesion of suspended cells. Int J Mol Med 16: 525-531, 2005
APA
DvoRánková, B., Smetana, K., Chovanec, M., Lacina, L., Stork, J., Plzáková, Z. ... Gabius, H. (2005). Transient expression of keratin 19 is induced in originally negative interfollicular epidermal cells by adhesion of suspended cells. International Journal of Molecular Medicine, 16, 525-531. https://doi.org/10.3892/ijmm.16.4.525
MLA
DvoRánková, B., Smetana, K., Chovanec, M., Lacina, L., Stork, J., Plzáková, Z., GaloviCová, M., Gabius, H."Transient expression of keratin 19 is induced in originally negative interfollicular epidermal cells by adhesion of suspended cells". International Journal of Molecular Medicine 16.4 (2005): 525-531.
Chicago
DvoRánková, B., Smetana, K., Chovanec, M., Lacina, L., Stork, J., Plzáková, Z., GaloviCová, M., Gabius, H."Transient expression of keratin 19 is induced in originally negative interfollicular epidermal cells by adhesion of suspended cells". International Journal of Molecular Medicine 16, no. 4 (2005): 525-531. https://doi.org/10.3892/ijmm.16.4.525