Expression pattern and subcellular localization of five splice isoforms of human PXK

  • Authors:
    • Xianqiong Zou
    • Guanzhou Qiu
    • Chunjing Chen
    • Maoqing Wu
    • Yuehua Hu
    • Huarui Zheng
    • Xin Li
    • Shaohua Gu
    • Chaoneng Ji
    • Yumin Mao
  • View Affiliations

  • Published online on: October 1, 2005     https://doi.org/10.3892/ijmm.16.4.701
  • Pages: 701-707
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Abstract

Phox homology (PX) domains specifically bind to phosphoinositides, and this interaction is crucial for their cellular function. A full-length cDNA of human PX domain containing serine/threonine kinase gene (PXK) that we termed PXK_v1 had previously been cloned. PXK_v1 consists of a S_TKc domain (serine/threonine kinases, catalytic domain), but lacks several residues that are indispensable for intrinsic catalytic activity. Evidence obtained in the present study demonstrated the existence of four other splice isoforms of human PXK in fetal brain, designated as PXK_v2, PXK_v3, PXK_v4 and PXK_v5. The results of RT-PCR indicated that human PXK_v1, PXK_v2 and PXK_v4 transcripts were widely expressed in human adult tissue, except heart tissue. In contrast, PXK_v3 transcripts were only expressed in peripheral blood leukocytes at a low level and PXK_v5 transcripts were not detectable in any of the tissue analyzed. Subcellular localization analysis of EGFP-PXK fusion proteins in COS7 cells indicated that EGFP-PXK_v3 had a different subcellular localization compared to other EGFP-PXK fusion proteins. Mutation analysis of EGFP-PXK_v1 showed that PXK_v1-Tyr56 and Arg92 are essential for subcellular localization of the protein in the cytoplasm.

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October 2005
Volume 16 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Zou X, Qiu G, Chen C, Wu M, Hu Y, Zheng H, Li X, Gu S, Ji C, Mao Y, Mao Y, et al: Expression pattern and subcellular localization of five splice isoforms of human PXK. Int J Mol Med 16: 701-707, 2005
APA
Zou, X., Qiu, G., Chen, C., Wu, M., Hu, Y., Zheng, H. ... Mao, Y. (2005). Expression pattern and subcellular localization of five splice isoforms of human PXK. International Journal of Molecular Medicine, 16, 701-707. https://doi.org/10.3892/ijmm.16.4.701
MLA
Zou, X., Qiu, G., Chen, C., Wu, M., Hu, Y., Zheng, H., Li, X., Gu, S., Ji, C., Mao, Y."Expression pattern and subcellular localization of five splice isoforms of human PXK". International Journal of Molecular Medicine 16.4 (2005): 701-707.
Chicago
Zou, X., Qiu, G., Chen, C., Wu, M., Hu, Y., Zheng, H., Li, X., Gu, S., Ji, C., Mao, Y."Expression pattern and subcellular localization of five splice isoforms of human PXK". International Journal of Molecular Medicine 16, no. 4 (2005): 701-707. https://doi.org/10.3892/ijmm.16.4.701