Expression of β-catenin is regulated by PI-3 kinase and sodium butyrate in colorectal cancer cells

  • Authors:
    • Jolana Turecková
    • Dana Kucerová
    • Martina Vojtechová
    • Eva Sloncová
    • Zdena Tuhácková
  • View Affiliations

  • Published online on: January 1, 2006     https://doi.org/10.3892/ijmm.17.1.69
  • Pages: 69-75
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Abstract

β-catenin has a dual function; it is implicated in intercellular junctions and transcriptional co-activation. Here we examined the regulation of the expression and localization of β-catenin in HT29 colorectal adenocarcinoma cells. Our results showed that inhibition of PI-3 kinase with wortmannin was accompanied by a considerably reduced expression of β-catenin. This effect was overcome by butyrate and occured at the protein level, not at the level of mRNA. Moreover, NaBT significantly increased the phosphorylation of the ribosomal protein, S6, known to participate in the translational control of gene expression. This was accompanied by the increased phosphorylation of p70 S6K and MAPKs, the effector proteins that are upstream of protein S6 in the distinct signaling pathways. These facts indicate that different signaling pathways may be involved in the regulation of β-catenin synthesis. Modulation of β-catenin expression induced by NaBT appeared to occur at the level of protein translation, suggesting that NaBT may act as a translational regulator.

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January 2006
Volume 17 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Turecková J, Kucerová D, Vojtechová M, Sloncová E and Tuhácková Z: Expression of β-catenin is regulated by PI-3 kinase and sodium butyrate in colorectal cancer cells. Int J Mol Med 17: 69-75, 2006
APA
Turecková, J., Kucerová, D., Vojtechová, M., Sloncová, E., & Tuhácková, Z. (2006). Expression of β-catenin is regulated by PI-3 kinase and sodium butyrate in colorectal cancer cells. International Journal of Molecular Medicine, 17, 69-75. https://doi.org/10.3892/ijmm.17.1.69
MLA
Turecková, J., Kucerová, D., Vojtechová, M., Sloncová, E., Tuhácková, Z."Expression of β-catenin is regulated by PI-3 kinase and sodium butyrate in colorectal cancer cells". International Journal of Molecular Medicine 17.1 (2006): 69-75.
Chicago
Turecková, J., Kucerová, D., Vojtechová, M., Sloncová, E., Tuhácková, Z."Expression of β-catenin is regulated by PI-3 kinase and sodium butyrate in colorectal cancer cells". International Journal of Molecular Medicine 17, no. 1 (2006): 69-75. https://doi.org/10.3892/ijmm.17.1.69