Anti-apoptotic hPEBP4 silencing promotes TRAIL-induced apoptosis of human ovarian cancer cells by activating ERK and JNK pathways

  • Authors:
    • Peng Li
    • Xiaojian Wang
    • Nan Li
    • Huabei Kong
    • Zhenhong Guo
    • Shuxun Liu
    • Xuetao Cao
  • View Affiliations

  • Published online on: September 1, 2006     https://doi.org/10.3892/ijmm.18.3.505
  • Pages: 505-510
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Abstract

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) induces apoptosis in a variety of tumor cell lines but not typically in normal or nontransformed cells, which makes TRAIL a desirable therapeutic agent to fight cancer. Human phosphatidylethanolamine-binding protein 4 (hPEBP4) is a recently identified anti-apoptotic molecule and has been shown to be highly expressed in breast and ovarian cancer cells. We demonstrate that silencing of hPEBP4 in CaoV-3 ovarian cancer cells potentiates TRAIL-induced apoptosis. We found that endogenous hPEBP4 interacts with Raf-1 and MEK1 in TRAIL-treated CaoV-3 cells by co-immunoprecipitation analysis. Simultaneously, silencing of hPEBP4 in CaoV-3 cells enhances TRAIL-induced ERK and JNK activation. Moreover, the inhibitors of MEK1 or JNK can reduce hPEBP4-silence-induced TRAIL sensitivity. Therefore, silencing of hPEBP4 in CaoV-3 ovarian cancer promotes TRAIL-induced apoptosis, and the increased MAPK activation is required for the apoptosis sensitization. All these data indicate that silencing of hPEBP4, an important potential target, may be a promising approach for the treatment of ovarian cancer.

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September 2006
Volume 18 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Li P, Wang X, Li N, Kong H, Guo Z, Liu S and Cao X: Anti-apoptotic hPEBP4 silencing promotes TRAIL-induced apoptosis of human ovarian cancer cells by activating ERK and JNK pathways. Int J Mol Med 18: 505-510, 2006
APA
Li, P., Wang, X., Li, N., Kong, H., Guo, Z., Liu, S., & Cao, X. (2006). Anti-apoptotic hPEBP4 silencing promotes TRAIL-induced apoptosis of human ovarian cancer cells by activating ERK and JNK pathways. International Journal of Molecular Medicine, 18, 505-510. https://doi.org/10.3892/ijmm.18.3.505
MLA
Li, P., Wang, X., Li, N., Kong, H., Guo, Z., Liu, S., Cao, X."Anti-apoptotic hPEBP4 silencing promotes TRAIL-induced apoptosis of human ovarian cancer cells by activating ERK and JNK pathways". International Journal of Molecular Medicine 18.3 (2006): 505-510.
Chicago
Li, P., Wang, X., Li, N., Kong, H., Guo, Z., Liu, S., Cao, X."Anti-apoptotic hPEBP4 silencing promotes TRAIL-induced apoptosis of human ovarian cancer cells by activating ERK and JNK pathways". International Journal of Molecular Medicine 18, no. 3 (2006): 505-510. https://doi.org/10.3892/ijmm.18.3.505