Conformationally altered hyaluronan restricts complement classical pathway activation by binding to C1q, C1r, C1s, C2, C5 and C9, and suppresses WOX1 expression in prostate DU145 cells

  • Authors:
    • Qunying Hong
    • Emory Kuo
    • Lori Schultz
    • Robert J. Boackle
    • Nan-Shan Chang
  • View Affiliations

  • Published online on: January 1, 2007     https://doi.org/10.3892/ijmm.19.1.173
  • Pages: 173-179
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Abstract

Linear non-sulfated hyaluronan (HA) does not bind complement proteins yet inhibits their hemolytic function. We have previously induced the complement inhibitory function of HA by heat treatment. However, heated HA readily loses its anti-complementary activity probably due to instantaneous interchain re-association. Here, HA solutions were heated and then freeze-dried. Compared to native HA, heated/freeze-dried HA stably restricted serum complement-mediated hemolysis via the classical pathway, in which serum C1 hemolytic function and C3 activation were blocked. Also, treated HA had a significantly increased binding of component C1q, C1r, C1s, C2, C5, C9, P, D and H. Further, when HA was gel-fractionated by electrophoresis and then freeze-dried, its anti-complementary activity was stably induced. Both native and heated/freeze-dried HA stimulated ERK phosphorylation in prostate DU145 cells. However, treated HA suppressed the expression of tumor suppressors WOX1 and WOX2. Together, HA with an altered conformation stabilizes its inhibition and binding of complement proteins. It may recognize cell surface receptors differently from native HA, thereby differentially regulating the expression of cellular proteins.

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January 2007
Volume 19 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Hong Q, Kuo E, Schultz L, Boackle RJ and Chang N: Conformationally altered hyaluronan restricts complement classical pathway activation by binding to C1q, C1r, C1s, C2, C5 and C9, and suppresses WOX1 expression in prostate DU145 cells. Int J Mol Med 19: 173-179, 2007
APA
Hong, Q., Kuo, E., Schultz, L., Boackle, R.J., & Chang, N. (2007). Conformationally altered hyaluronan restricts complement classical pathway activation by binding to C1q, C1r, C1s, C2, C5 and C9, and suppresses WOX1 expression in prostate DU145 cells. International Journal of Molecular Medicine, 19, 173-179. https://doi.org/10.3892/ijmm.19.1.173
MLA
Hong, Q., Kuo, E., Schultz, L., Boackle, R. J., Chang, N."Conformationally altered hyaluronan restricts complement classical pathway activation by binding to C1q, C1r, C1s, C2, C5 and C9, and suppresses WOX1 expression in prostate DU145 cells". International Journal of Molecular Medicine 19.1 (2007): 173-179.
Chicago
Hong, Q., Kuo, E., Schultz, L., Boackle, R. J., Chang, N."Conformationally altered hyaluronan restricts complement classical pathway activation by binding to C1q, C1r, C1s, C2, C5 and C9, and suppresses WOX1 expression in prostate DU145 cells". International Journal of Molecular Medicine 19, no. 1 (2007): 173-179. https://doi.org/10.3892/ijmm.19.1.173