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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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January 2007 Volume 19 Issue 1

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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January 2007 Volume 19 Issue 1

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Article

Significant change in insulin production, glucose tolerance and ER stress signaling in transgenic mice coexpressing insulin-siRNA and human IDE

  • Authors:
    • Dae Youn Hwang
    • Sujin Seo
    • Yongkyu Kim
    • Chuelkyu Kim
    • Sunbo Shim
    • Seungwan Jee
    • Suhae Lee
    • Jisoon Sin
    • Junyong Cho
    • Byeongchuel Kang
    • Insurk Jang
    • Jungsik Cho
  • View Affiliations / Copyright

    Affiliations: Laboratory Animal Resources Team, National Institute of Toxicological Research, Korea FDA, Seoul 122-704, Korea. dyhwang@kfda.go.kr
  • Pages: 65-73
    |
    Published online on: January 1, 2007
       https://doi.org/10.3892/ijmm.19.1.65
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Abstract

The dual expression system for the suppression and clearance of insulin has not been previously used to produce transgenic mice for diabetes-related disease. The aim of this study was to produce new transgenic mice coexpressing specific insulin small interfering RNA (siRNA) sequences and the human insulin degrading enzyme (hIDE) gene in order to examine the diabetes-like phenotype. To achieve this, a new lineage of transgenic mice was produced by the microinjection of the dual expression constructs (pH1/siRNAinsulin-CMV/hIDE) into mouse fertilized eggs. The results showed that overexpressing the insulin siRNA and hIDE genes resulted in the induction of the human enzyme, impaired glucose tolerance and lower serum insulin levels compared to the Non-Tg mice. Moreover, the Tg mice aged 20 weeks had a significantly activated ER stress signaling compared to their Non-Tg counterparts, which may be associated with the suppression of insulin production in the pancreas and the degradation of insulin in the liver, respectively. Therefore, insulin-suppressed transgenic mice can be used to examine diabetes as a new diabetes-like phenotype model, which results in a lower level of circulating insulin without the destruction of pancreatic islets.

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Copy and paste a formatted citation
Spandidos Publications style
Hwang DY, Seo S, Kim Y, Kim C, Shim S, Jee S, Lee S, Sin J, Cho J, Kang B, Kang B, et al: Significant change in insulin production, glucose tolerance and ER stress signaling in transgenic mice coexpressing insulin-siRNA and human IDE. Int J Mol Med 19: 65-73, 2007.
APA
Hwang, D.Y., Seo, S., Kim, Y., Kim, C., Shim, S., Jee, S. ... Cho, J. (2007). Significant change in insulin production, glucose tolerance and ER stress signaling in transgenic mice coexpressing insulin-siRNA and human IDE. International Journal of Molecular Medicine, 19, 65-73. https://doi.org/10.3892/ijmm.19.1.65
MLA
Hwang, D. Y., Seo, S., Kim, Y., Kim, C., Shim, S., Jee, S., Lee, S., Sin, J., Cho, J., Kang, B., Jang, I., Cho, J."Significant change in insulin production, glucose tolerance and ER stress signaling in transgenic mice coexpressing insulin-siRNA and human IDE". International Journal of Molecular Medicine 19.1 (2007): 65-73.
Chicago
Hwang, D. Y., Seo, S., Kim, Y., Kim, C., Shim, S., Jee, S., Lee, S., Sin, J., Cho, J., Kang, B., Jang, I., Cho, J."Significant change in insulin production, glucose tolerance and ER stress signaling in transgenic mice coexpressing insulin-siRNA and human IDE". International Journal of Molecular Medicine 19, no. 1 (2007): 65-73. https://doi.org/10.3892/ijmm.19.1.65
Copy and paste a formatted citation
x
Spandidos Publications style
Hwang DY, Seo S, Kim Y, Kim C, Shim S, Jee S, Lee S, Sin J, Cho J, Kang B, Kang B, et al: Significant change in insulin production, glucose tolerance and ER stress signaling in transgenic mice coexpressing insulin-siRNA and human IDE. Int J Mol Med 19: 65-73, 2007.
APA
Hwang, D.Y., Seo, S., Kim, Y., Kim, C., Shim, S., Jee, S. ... Cho, J. (2007). Significant change in insulin production, glucose tolerance and ER stress signaling in transgenic mice coexpressing insulin-siRNA and human IDE. International Journal of Molecular Medicine, 19, 65-73. https://doi.org/10.3892/ijmm.19.1.65
MLA
Hwang, D. Y., Seo, S., Kim, Y., Kim, C., Shim, S., Jee, S., Lee, S., Sin, J., Cho, J., Kang, B., Jang, I., Cho, J."Significant change in insulin production, glucose tolerance and ER stress signaling in transgenic mice coexpressing insulin-siRNA and human IDE". International Journal of Molecular Medicine 19.1 (2007): 65-73.
Chicago
Hwang, D. Y., Seo, S., Kim, Y., Kim, C., Shim, S., Jee, S., Lee, S., Sin, J., Cho, J., Kang, B., Jang, I., Cho, J."Significant change in insulin production, glucose tolerance and ER stress signaling in transgenic mice coexpressing insulin-siRNA and human IDE". International Journal of Molecular Medicine 19, no. 1 (2007): 65-73. https://doi.org/10.3892/ijmm.19.1.65
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